MTCO2 Rekombinanter Antikörper

• Featured Product
• KD/KO Validated

MTCO2 Rekombinant Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

230448C9

Kat-Nr. : 83132-7-PBS

Synonyme

COX2, 230448C9, COII, COX 2, COXII

---

• 

  WB analysis using 83132-7-RR (same clone as 83132-7-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83132-7-RR (MTCO2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83132-7-PBS in a different storage buffer formulation.

• 

  WB analysis using 83132-7-RR (same clone as 83132-7-PBS)

  WB result of MTCO2 antibody (MTCO2; 1:3000; incubated at room temperature for 1.5 hours) with 143B and 143B.TK.ρ0 cell. 143B is wild-type cell line, 143B.TK.ρ0 is mtDNA deletion cell line. This data was developed using the same antibody clone with 83132-7-PBS in a different storage buffer formulation.

• 

  IP experiment of HeLa using 83132-7-RR (same clone as 83132-7-PBS)

  IP result of anti-MTCO2 (IP:83132-7-RR, 4ug; Detection:83132-7-RR 1:3000) with HeLa cells lysate 1750 ug. This data was developed using the same antibody clone with 83132-7-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovarian cancer using 83132-7-RR (same clone as 83132-7-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83132-7-RR (MTCO2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83132-7-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 83132-7-RR (same clone as 83132-7-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83132-7-RR (MTCO2 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9). This data was developed using the same antibody clone with 83132-7-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 83132-7-RR (same clone as 83132-7-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MTCO2 antibody (83132-7-RR, Clone: 230448C9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83132-7-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 83132-7-RR (same clone as 83132-7-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Anti-Human MTCO2 (83132-7-RR, Clone:230448C9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83132-7-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

83132-7-PBS bindet in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA MTCO2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: cytochrome c oxidase II
• Berechnetes Molekulargewicht: 26 kDa
• Beobachtetes Molekulargewicht: 23-26 kDa
• GenBank-Zugangsnummer: YP_003024029
• Gene symbol: MTCO2
• Gene ID (NCBI): 4513
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MTCO2, also named as COII, COXII, and COX2, belongs to the cytochrome c oxidase subunit 2 family. It is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1. Defects in COX2 are a cause of mitochondrial complex IV deficiency (MT-C4D).