MTCO3 Polyklonaler Antikörper

MTCO3 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

14

Kat-Nr. : 55082-1-AP

Synonyme

COIII, COXIII, cytochrome c oxidase III, Cytochrome c oxidase subunit 3, MT CO3, MTCO3

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Galerie der Validierungsdaten

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  WB analysis using 55082-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55082-1-AP (MTCO3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of C2C12 using 55082-1-AP

  C2C12 cells were subjected to SDS PAGE followed by western blot with 55082-1-AP (MTCO3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 55082-1-AP

  IP Result of anti-MTCO3 (IP:55082-1-AP, 4ug; Detection:55082-1-AP 1:300) with mouse heart tissue lysate 4000ug.

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  IHC staining of mouse cerebellum using 55082-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 55082-1-AP (MTCO3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 55082-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 55082-1-AP (MTCO3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 55082-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 55082-1-AP (MTCO3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 55082-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 55082-1-AP (MTCO3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 55082-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 55082-1-AP (MTCO3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausnierengewebe, C2C12-Zellen, Mauslebergewebe, Rattenlebergewebe, Rattennierengewebe
• Erfolgreiche IP: Mausherzgewebe
• Erfolgreiche Detektion in IHC: Maus-Cerebellum-Gewebe, Mausnierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 13 publications below
• IF: See 2 publications below

Produktinformation

55082-1-AP bindet in WB, IP, IHC, IF, ELISA MTCO3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: cytochrome c oxidase III
• Berechnetes Molekulargewicht: 30 kDa
• Beobachtetes Molekulargewicht: 32-35 kDa
• GenBank-Zugangsnummer: YP_003024032
• Gene symbol: COX3
• Gene ID (NCBI): 4514
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MTCO3 (cytochrome c oxidase subunit III) is a mitochondrial DNA encoded subunit of respiratory Complex IV which is located within the mitochondrial inner membrane and is the final enzyme of the electron transport chain of mitochondrial oxidative phosphorylation. The predicted MW of MTCO3 is 30 kDa, while it had been observed as 23 kDa in some papers (PMID: 17557076).

Publikationen

Species	Application	Title
mouse	WB	Cell Res; Active RNA interference in mitochondria.; Authors - Kuanxing Gao
mouse	WB	Diabetes; Transketolase Deficiency in Adipose Tissues Protects Mice From Diet-Induced Obesity by Promoting Lipolysis.; Authors - Na Tian
mouse	WB	Int J Mol Sci; Mitochondrial rRNA Methylation by Mettl15 Contributes to the Exercise and Learning Capability in Mice.; Authors - Olga A Averina
human	WB	J Med Genet; COA7 (C1orf163/RESA1) mutations associated with mitochondrial leukoencephalopathy and cytochrome c oxidase deficiency.; Authors - Anabel Martinez Lyons
mouse	IF	Sci China Life Sci; Cholesterol homeostasis regulated by ABCA1 is critical for retinal ganglion cell survival.; Authors - Jialiang Yang
human	WB	Carcinogenesis; Mitochondrial miR-181a-5p promotes glucose metabolism reprogramming in liver cancer by regulating the electron transport chain.; Authors - Xiang Zhuang