MTH1 Polyklonaler Antikörper

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• KD/KO Validated

MTH1 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16705-1-PBS

Synonyme

NUDT1, 2-hydroxy-dATP diphosphatase, 7,8-dihydro-8-oxoguanine triphosphatase, 8 oxo dGTPase, 8-oxo-dGTPase

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  WB analysis of Jurkat using 16705-1-AP (same clone as 16705-1-PBS)

  WB result of MTH1 antibody (16705-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MTH1 transfected Jurkat cells. This data was developed using the same antibody clone with 16705-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 16705-1-AP (same clone as 16705-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16705-1-AP (MTH1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16705-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 16705-1-AP (same clone as 16705-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16705-1-AP (MTH1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16705-1-PBS in a different storage buffer formulation.

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  WB analysis of PC-3 using 16705-1-AP (same clone as 16705-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 16705-1-AP (MTH1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16705-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 16705-1-AP (same clone as 16705-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16705-1-AP (MTH1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16705-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 16705-1-AP (same clone as 16705-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16705-1-AP (MTH1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16705-1-PBS in a different storage buffer formulation.

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  IF Staining of PC-3 using 16705-1-AP (same clone as 16705-1-PBS)

  Immunofluorescent analysis of PC-3 cells using 16705-1-AP (MTH1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16705-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

16705-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA MTH1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MTH1 fusion protein Ag10103
• Vollständiger Name: nudix (nucleoside diphosphate linked moiety X)-type motif 1
• Berechnetes Molekulargewicht: 179aa,20 kDa; 197aa,23 kDa
• Beobachtetes Molekulargewicht: 18-24 kDa
• GenBank-Zugangsnummer: BC014618
• Gene symbol: MTH1
• Gene ID (NCBI): 4521
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MTH1, also known as NUDT1 or 8-Oxo-dGTPase, is an important repair enzyme that prevents oxidative stress-induced DNA damage through hydrolyzing mutagenic 8-oxo-dGTP into 8-oxo-dGMP. MTH1 is expressed in postmitotic neurons as well as in proliferative tissues, and it is localized both in the mitochondria and nucleus. MTH1 might be a useful marker of oxidative stress and its level increased upon oxidative stress. Increased expression of MTH1 were also found in various cancer tissues and neurodegenerative diseases.