MTHFD1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

MTHFD1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(15)

Rezensionen (1)

Kat-Nr. : 10794-1-AP

Synonyme

C-1-tetrahydrofolate synthase, cytoplasmic, C-1-tetrahydrofolate synthase, cytoplasmic, N-terminally processed, C1-THF synthase, Epididymis secretory sperm binding protein, MTHFC

---

• 

  WB analysis using 10794-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10794-1-AP (MTHFD1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10794-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10794-1-AP (MTHFD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 10794-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10794-1-AP (MTHFD1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10794-1-AP

  HuH-7 cells were subjected to SDS PAGE followed by western blot with 10794-1-AP (MTHFD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse kidney using 10794-1-AP

  IP result of anti-MTHFD1 (IP:10794-1-AP, 5ug; Detection:10794-1-AP 1:600) with mouse kidney tissue lysate 4000ug.

• 

  IHC staining of human liver cancer using 10794-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10794-1-AP (MTHFD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 10794-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10794-1-AP (MTHFD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 10794-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10794-1-AP (MTHFD1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 10794-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10794-1-AP (MTHFD1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10794-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10794-1-AP (MTHFD1 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 10794-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10794-1-AP (MTHFD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 10794-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10794-1-AP (MTHFD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10794-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10794-1-AP (MTHFD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10794-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10794-1-AP (MTHFD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HEK-293 using 10794-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using MTHFD1 antibody (10794-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HuH-7-Zellen, humanes Hirngewebe, K-562-Zellen, MCF-7-Zellen, Mausnierengewebe
• Erfolgreiche IP: Mausnierengewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Kolonkarzinomgewebe, Mauslebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 14 publications below
• IHC: See 1 publications below
• IP: See 1 publications below

Produktinformation

10794-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA MTHFD1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MTHFD1 fusion protein Ag1225
• Vollständiger Name: methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase
• Berechnetes Molekulargewicht: 101 kDa
• Beobachtetes Molekulargewicht: 101 kDa
• GenBank-Zugangsnummer: BC009806
• Gene symbol: MTHFD1
• Gene ID (NCBI): 4522
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MTHFD1 is a trifunctional enzyme involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism. MTHFD1 is associated with HCC and CRC progression. It has been reported that MTHFD-deficient patient fibroblasts exhibit enriched MTHFD1 in the nucleus which is critical for de novo dTMP synthesis.(PMID: 31133746, 30997850, 31421459, 25548164)

Publikationen

Species	Application	Title
human	WB	Nature; Oxidative stress inhibits distant metastasis by human melanoma cells.; Authors - Elena Piskounova ; KD Validated
human	WB	J Pineal Res; Melatonin modulates metabolic remodeling in HNSCC by suppressing MTHFD1L-formate axis.; Authors - Li Cui
human	WB	Redox Biol; Cytosolic ME1 integrated with mitochondrial IDH2 supports tumor growth and metastasis.; Authors - Chang Shao
human	WB	Proc Natl Acad Sci U S A; Orthogonal genome-wide screens of bat cells identify MTHFD1 as a target of broad antiviral therapy.; Authors - Danielle E Anderson ; KD Validated
mouse	WB	Dev Cell; Illuminating NAD+ Metabolism in Live Cells and In Vivo Using a Genetically Encoded Fluorescent Sensor.; Authors - Yejun Zou
human	WB	Cell Death Dis; MTHFD1 regulates the NADPH redox homeostasis in MYCN-amplified neuroblastoma; Authors - Jinqiu Guan

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Hua (Verified Customer) (08-26-2019)

Works well.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: liver, kidney, Hippocampus

MTHFD1 Antibody Western Blot, validation (1:500 dilution) in liver, kidney, Hippocampus (Cat no:10794-1-AP)