MTHFD1L Monoklonaler Antikörper

MTHFD1L Monoklonal Antikörper für WB, IHC, FC (Intra), Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Kaninchen, Maus, Ratte

Anwendung

WB, IHC, FC (Intra), Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1G1F2

Kat-Nr. : 68321-1-PBS

Synonyme

MTC1THFS, Monofunctional C1-tetrahydrofolate synthase, mitochondrial, FTHFSDC1, EC:6.3.4.3, 1G1F2

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  WB analysis using 68321-1-Ig (same clone as 68321-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68321-1-Ig (MTHFD1L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 68321-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68321-1-Ig (same clone as 68321-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68321-1-Ig (MTHFD1L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 68321-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 68321-1-Ig (same clone as 68321-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68321-1-Ig (MTHFD1L antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68321-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 68321-1-Ig (same clone as 68321-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68321-1-Ig (MTHFD1L antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68321-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 68321-1-Ig (same clone as 68321-1-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.4 ug MTHFD1L Monoclonal antibody (68321-1-Ig, Clone:1G1F2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG2b isotype control Mouse McAb (66360-3-Ig, Clone: 11B8C4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68321-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50271-2

  Cytometric bead array standard curve of MP50271-2, MTHFD1L Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68321-1-PBS. Detection antibody: 68321-3-PBS. Standard:Ag9061. Range: 0.195-50 ng/mL.

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Geprüfte Anwendungen

Produktinformation

68321-1-PBS bindet in WB, IHC, FC (Intra), Cytometric bead array, Indirect ELISA MTHFD1L und zeigt Reaktivität mit human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: MTHFD1L fusion protein Ag9061
• Vollständiger Name: methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like
• Berechnetes Molekulargewicht: 978 aa, 106 kDa
• Beobachtetes Molekulargewicht: 106 kDa
• GenBank-Zugangsnummer: BC017477
• Gene symbol: MTHFD1L
• Gene ID (NCBI): 25902
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MTHFD1L(Monofunctional C1-tetrahydrofolate synthase, mitochondrial) is also named as FTHFSDC1(Formyltetrahydrofolate synthetase). MTHFD1L enzyme is present in mitochondria from normal embryonic tissues and embryonic fibroblast cell lines, and embryonic mitochondria possess the ability to synthesize formate from glycine. It catalyzes the final step in the mitochondrial conversion of 1-C units to formate in embryos. Moreover, MTHFD1L levels were substantially higher in embryonic mitochondria than in adult liver mitochondria and embryonic mitochondria exhibited greater formate production(PMID:19948730). It has 2 isoforms produced by alternative splicing.