MTHFD2 Monoklonaler Antikörper

MTHFD2 Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1B4B6

Kat-Nr. : 68524-1-PBS

Synonyme

MTHFD2

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  WB analysis using 68524-1-Ig (same clone as 68524-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68524-1-Ig (MTHFD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68524-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 68524-1-Ig (same clone as 68524-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68524-1-Ig (MTHFD2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68524-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 68524-1-Ig (same clone as 68524-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68524-1-Ig (MTHFD2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68524-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 68524-1-Ig (same clone as 68524-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68524-1-Ig (MTHFD2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68524-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 68524-1-Ig (same clone as 68524-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68524-1-Ig (MTHFD2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68524-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68524-1-PBS bindet in WB, IHC, Indirect ELISA MTHFD2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: MTHFD2 fusion protein Ag33605
• Vollständiger Name: methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase
• Berechnetes Molekulargewicht: 350 aa, 38 kDa
• Beobachtetes Molekulargewicht: 33-38 kDa
• GenBank-Zugangsnummer: BC017054
• Gene symbol: MTHFD2
• Gene ID (NCBI): 10797
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Methylenetetrahydrofolate dehydrogenase 2 (MTHFD2) is a folate-coupled mitochondrial metabolic enzyme characterized by methylenetetrahydrofolate dehydrogenase and cyclohydrolase activity. It was demonstrated that suppression of MTHFD2 inhibits methylation reactions, decreases protein synthesis and disrupts redox homeostasis, which may ultimately result in significant changes in cellular metabolic phenotype. MTHFD2 is reported to be up‐regulated in various tumours, such as lung adenocarcinoma(PMID: 34121323) and breast cancer(PMID: 34007312).