MTHFR Rekombinanter Antikörper

MTHFR Rekombinant Antikörper für Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

240348H7

Kat-Nr. : 83372-4-PBS

Synonyme

EC:1.5.1.53, Methylenetetrahydrofolate reductase (NADPH)

---

• 

  Sandwich ELISA standard curve of MP00372-1

  Sandwich ELISA standard curve of MP00372-1, Human MTHFR Recombinant Matched Antibody Pair - PBS only. 83372-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag14970. 83372-4-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL

• 

  Sample test of MP00372-1

  K-562, Hela and HepG2 cell lysates were measured. The human MTHFR concentration of detected samples was determined to be 88.86 ng/mL (based on a 4.5 mg/mL extract load) in K-562 cell lysate, 57.89 ng/mL (based on a 3.2 mg/mL extract load) in Hela cell lysate and 55.55 ng/mL (based on a 5 mg/mL extract load) in HepG2 cell lysate.

• 

  Cytometric bead array standard curve of MP00372-1

  Cytometric bead array standard curve of MP00372-1, MTHFR Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83372-5-PBS. Detection antibody: 83372-4-PBS. Standard: Ag14970. Range: 0.78-100 ng/mL.

• 

  Affinity and Kinetic Characterization of 83372-4-PBS

  Biolayer interferometry (BLl) kinetic assay of 83372-4-PBS against Human MTHFR was performed. The affinity constant is 0.759 nM.

---

Geprüfte Anwendungen

Produktinformation

83372-4-PBS bindet in Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test MTHFR und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: MTHFR fusion protein Ag14970
• Vollständiger Name: 5,10-methylenetetrahydrofolate reductase (NADPH)
• Berechnetes Molekulargewicht: 656 aa, 75 kDa
• GenBank-Zugangsnummer: BC053509
• Gene symbol: MTHFR
• Gene ID (NCBI): 4524
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.