MTHFR Monoklonaler Antikörper

MTHFR Monoklonal Antikörper für IF/ICC

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

IF/ICC

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

1F7A3

Kat-Nr. : CL594-66612

Synonyme

Methylenetetrahydrofolate reductase (NADPH), EC:1.5.1.53, 1F7A3

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  IF Staining of HeLa using CL594-66612

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®594 MTHFR antibody (CL594-66612, Clone: 1F7A3 ) at dilution of 1:200.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL594-66612 bindet in IF/ICC MTHFR und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: MTHFR fusion protein Ag14970
• Vollständiger Name: 5,10-methylenetetrahydrofolate reductase (NADPH)
• Berechnetes Molekulargewicht: 656 aa, 75 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC053509
• Gene symbol: MTHFR
• Gene ID (NCBI): 4524
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

5,10-Methylenetetrahydrofolate reductase (MTHFR) is a crucial enzyme in the folate metabolic pathway with a key role in generating methyl groups. MTHFR reduces 5,10-methylenetetrahydrofolate (5,10-methyleneTHF) to 5-methylTHF, the primary carbon donor for methionine production from homocysteine. In humans and mice studies, mutations and polymorphisms in MTHFR are associated with impaired spermatogenesis and male infertility. It has been reported that MTHFR is highly phosphorylated under unperturbed conditions and T34 is the priming phosphorylation site. MTHFR gene has two promoters and isoform (70 kDa and 77 kDa). Although the major form of MTHFR is 77kDa in most tissues, a second isoform of 70kDa was observed after western blotting of human fetal liver and porcine liver. (PMID: 24769206, PMID: 34128976, PMID: 18615588)