MTHFS Polyklonaler Antikörper

MTHFS Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Rezensionen (1)

Kat-Nr. : 13114-1-AP

Synonyme

Methenyl-THF synthetase, Methenyl THF synthetase, HsT19268, EC:6.3.3.2, 5-formyltetrahydrofolate cyclo-ligase

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  WB analysis using 13114-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13114-1-AP (MTHFS antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 13114-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 13114-1-AP (MTHFS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13114-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13114-1-AP (MTHFS antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 13114-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13114-1-AP (MTHFS antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of human stomach using 13114-1-AP

  human stomach tissue were subjected to SDS PAGE followed by western blot with 13114-1-AP (MTHFS antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-937 using 13114-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 13114-1-AP (MTHFS antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 13114-1-AP

  IP result of anti-MTHFS (IP:13114-1-AP, 4ug; Detection:13114-1-AP 1:2000) with HeLa cells lysate 1200 ug.

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  IHC staining of human liver cancer using 13114-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 13114-1-AP (MTHFS antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver cancer using 13114-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 13114-1-AP (MTHFS antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human liver cancer using 13114-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 13114-1-AP (MTHFS antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver cancer using 13114-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 13114-1-AP (MTHFS antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, humanes Lebergewebe, humanes Magengewebe, Jurkat-Zellen, L02-Zellen, U-937-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

13114-1-AP bindet in WB, IHC, IP, ELISA MTHFS und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MTHFS fusion protein Ag3781
• Vollständiger Name: 5,10-methenyltetrahydrofolate synthetase (5-formyltetrahydrofolate cyclo-ligase)
• Berechnetes Molekulargewicht: 203 aa, 23 kDa
• Beobachtetes Molekulargewicht: 23 kDa
• GenBank-Zugangsnummer: BC019921
• Gene symbol: MTHFS
• Gene ID (NCBI): 10588
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Hua (Verified Customer) (11-14-2024)

Works fine for WB.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Moues liver

MTHFS Antibody Western Blot validation (1:2000 dilution) in Moues liver (Cat no:13114-1-AP)