MTMR12 Polyklonaler Antikörper

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• KD/KO Validated

MTMR12 Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 17552-1-AP

Synonyme

KIAA1682, Inactive phosphatidylinositol 3-phosphatase 12, 3-phosphatase adapter protein, 3-PAP, 3 phosphatase adapter protein

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  WB analysis using 17552-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 17552-1-AP (MTMR12 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17552-1-AP

  WB result of MTMR12 antibody (17552-1-AP; 1:400; room temperature for 1.5 hours) with wild-type and MTMR12 knockout HeLa cells.

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  WB analysis of HEK-293 using 17552-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17552-1-AP (MTMR12 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 17552-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 17552-1-AP (MTMR12 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 17552-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 17552-1-AP (MTMR12 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 17552-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17552-1-AP (MTMR12 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 17552-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 17552-1-AP (MTMR12 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17552-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17552-1-AP (MTMR12 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 17552-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17552-1-AP (MTMR12 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 17552-1-AP

  IP result of anti-MTMR12 (IP:17552-1-AP, 4ug; Detection:17552-1-AP 1:500) with K-562 cells lysate 2400 ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, HEK-293-Zellen, HeLa-Zellen, Maushirngewebe, Mauslungengewebe
• Erfolgreiche IP: K-562-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IP: See 1 publications below

Produktinformation

17552-1-AP bindet in WB, IP, ELISA MTMR12 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MTMR12 fusion protein Ag11710
• Vollständiger Name: myotubularin related protein 12
• Berechnetes Molekulargewicht: 86 kDa
• Beobachtetes Molekulargewicht: 73 kDa
• GenBank-Zugangsnummer: BC057393
• Gene symbol: MTMR12
• Gene ID (NCBI): 54545
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB,IP	FASEB Bioadv; In search of alternatively spliced alpha-Klotho Kl1 protein in mouse brain; Authors - Liping Li
	WB	BMC Urol; Effect of CTP-mediated PTEN on 5637 bladder cancer cells and the underlying molecular mechanism; Authors - Bei Yu