MTMR14 Polyklonaler Antikörper

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• KD/KO Validated

MTMR14 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 14973-1-AP

Synonyme

Myotubularin-related protein 14, hJumpy, hEDTP, HCV NS5A-transactivated protein 4 splice variant A-binding protein 1, EC:3.1.3.-

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  WB analysis using 14973-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14973-1-AP (MTMR14 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 14973-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using MTMR14 antibody (14973-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, Maushodengewebe, Rattenhodengewebe, RAW 264.7-Zellen
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below

Produktinformation

14973-1-AP bindet in WB, IF/ICC, ELISA MTMR14 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MTMR14 fusion protein Ag6883
• Vollständiger Name: myotubularin related protein 14
• Berechnetes Molekulargewicht: 72 kDa
• Beobachtetes Molekulargewicht: 60-72 kDa
• GenBank-Zugangsnummer: BC050626
• Gene symbol: MTMR14
• Gene ID (NCBI): 64419
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MTMR14, also known as Jumpy, belongs to the MTM1/MTMRs family which has 15 members that divided into two subgroup: catalytically inactive and active. MTMR14 is a catalytically active member possessing an active phosphatase domain, that specifically dephosphorylates PI3P and phosphatidylinositol (3,5)-bisphosphate (PI3,5P2) at position 3 on inositol ring. A remarkable feature of the MTM1/MTMRs family is the association between a catalytically inactive member and a catalytically active member, which plays an important regulatory role. MTMR14 has 3 isoforms with molecular weight of 60-72 kDa.

Publikationen

Species	Application	Title
human	WB	Gene; Knockdown MTMR14 promotes cell apoptosis and inhibits migration in liver cancer cells.; Authors - Zhaodong Li ; KD Validated