MTMR14 Monoklonaler Antikörper

MTMR14 Monoklonal Antikörper für WB, IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1A8B2

Kat-Nr. : 67561-1-PBS

Synonyme

C3orf29, FLJ22405, FLJ46453, FLJ90311, hEDTP, HJUMPY, MTMR14, NS5ATP4ABP1

---

• 

  WB analysis using 67561-1-Ig (same clone as 67561-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67561-1-Ig (MTMR14 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67561-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 67561-1-Ig (same clone as 67561-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 67561-1-Ig (MTMR14 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67561-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 67561-1-Ig (same clone as 67561-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67561-1-Ig (MTMR14 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67561-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse heart using 67561-1-Ig (same clone as 67561-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using MTMR14 antibody (67561-1-Ig, Clone: 1A8B2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67561-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

67561-1-PBS bindet in WB, IHC, IF-P, Indirect ELISA MTMR14 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: MTMR14 fusion protein Ag6883
• Vollständiger Name: myotubularin related protein 14
• Berechnetes Molekulargewicht: 72 kDa
• Beobachtetes Molekulargewicht: 74 kDa
• GenBank-Zugangsnummer: BC050626
• Gene symbol: MTMR14
• Gene ID (NCBI): 64419
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MTMR14, also known as Jumpy, belongs to the MTM1/MTMRs family which has 15 members that divided into two subgroup: catalytically inactive and active. MTMR14 is a catalytically active member possessing an active phosphatase domain, that specifically dephosphorylates PI3P and phosphatidylinositol (3,5)-bisphosphate (PI3,5P2) at position 3 on inositol ring. A remarkable feature of the MTM1/MTMRs family is the association between a catalytically inactive member and a catalytically active member, which plays an important regulatory role. MTMR14 has 3 isoforms with molecular weight of 60-72 kDa, and also can be detected as a higher molecular weight due to the heterodimerization with other MTMR members.