MTMR15/FAN1 Polyklonaler Antikörper

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• KD/KO Validated

MTMR15/FAN1 Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Rezensionen (1)

Kat-Nr. : 17600-1-AP

Synonyme

MTMR15, EC:3.1.21.-, FAN1, FANCD2/FANCI-associated nuclease 1, Fanconi-associated nuclease 1

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  WB analysis using 17600-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17600-1-AP (MTMR15 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human spleen using 17600-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 17600-1-AP (MTMR15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 17600-1-AP

  IP result of anti-MTMR15 (IP:17600-1-AP, 4ug; Detection:17600-1-AP 1:300) with HeLa cells lysate 2800 ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, humanes Milzgewebe
• Erfolgreiche IP: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IP: See 1 publications below

Produktinformation

17600-1-AP bindet in WB, IP, ELISA MTMR15/FAN1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MTMR15/FAN1 fusion protein Ag11651
• Vollständiger Name: myotubularin related protein 15
• Berechnetes Molekulargewicht: 60 kDa, 114 kDa
• Beobachtetes Molekulargewicht: 63-68 kDa, 120-140 kDa
• GenBank-Zugangsnummer: BC047882
• Gene symbol: MTMR15
• Gene ID (NCBI): 22909
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MTMR15, also named as FAN1 and KIAA1018, is a nuclease required for the repair of DNA interstrand cross-links (ICL) recruited at sites of DNA damage by monoubiquitinated FANCD2. It is specifically involved in repair of ICL-induced DNA breaks by being required for efficient homologous recombination, probably in the resolution of homologous recombination intermediates. MTMR15 has some isoforms with MW 114 kDa and 60 kDa. The MW will be migrated to 120-140 kDa and 63-68 kDa with modification.

Publikationen

Species	Application	Title
	WB	Nat Struct Mol Biol; Fanconi anemia proteins participate in a break-induced-replication-like pathway to counter replication stress.; Authors - Xinlin Xu
human	WB,IP	Genes Cells; MLH1-mediated recruitment of FAN1 to chromatin for the induction of apoptosis triggered by O6 -methylguanine.; Authors - Mihoko Rikitake ; KD Validated
human	WB	Cell Rep; HIV-1 exploits the Fanconi anemia pathway for viral DNA integration.; Authors - Shaozu Fu
human	WB	Nat Commun; Structural and molecular basis of PCNA-activated FAN1 nuclease function in DNA repair; Authors - F Li

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Alexandra (Verified Customer) (07-04-2024)

Detectable both with endogenous and overexpressed protein from plasmid. One of the two bands ran in between the two expected molecular weights, the other was at the proper size. It is usable for the examination of the effect of endogenous gene silencing and for overexpression as well. Possibly higher dilution and longer incubation with the antibody is enough.

• Applications: Western Blot, Immunofluorescence, Cell culture
• Primary Antibody Dilution: 1000x
• Cell Tissue Type: U2OS human osteosarcoma cell line

MTMR15/FAN1 Antibody Western Blot,Immunofluorescence,Cell culture validation (1000x dilution) in U2OS human osteosarcoma cell line (Cat no:17600-1-AP)