MTMR15 Rekombinanter Antikörper

MTMR15 Rekombinant Antikörper für FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

240629E4

Kat-Nr. : 83622-6-RR

Synonyme

hFAN1, Fanconi-associated nuclease 1, FANCD2/FANCI-associated nuclease 1, FAN1, EC:3.1.21.-

---

• 

  FC experiment of K-562 using 83622-6-RR

  1x10^6 K-562 cells were intracellularly stained with 0.25 ug Mtmr15 Recombinant Antibody (83622-6-RR, Clone:240629E4) (red), or 0.25 ug Isotype Control (blue) and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

• 

  FC experiment of A431 using 83622-6-RR

  1x10^6 A431 cells were intracellularly stained with 0.25 ug Mtmr15 Recombinant Antibody (83622-6-RR, Clone:240629E4)(red), or 0.25 ug Isotype Control (blue) and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

• 

  Affinity and Kinetic Characterization of 83622-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83622-6-RR against Human MTMR15 were performed. The affinity constant is 3.74 nM.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in FC (Intra): K-562-Zellen, A431-Zellen

Empfohlene Verdünnung

• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83622-6-RR bindet in FC (Intra), ELISA MTMR15 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: MTMR15 fusion protein Ag11651
• Vollständiger Name: myotubularin related protein 15
• Berechnetes Molekulargewicht: 60 kDa, 114 kDa
• GenBank-Zugangsnummer: BC047882
• Gene symbol: MTMR15
• Gene ID (NCBI): 22909
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MTMR15, also named as FAN1 and KIAA1018, is a nuclease required for the repair of DNA interstrand cross-links (ICL) recruited at sites of DNA damage by monoubiquitinated FANCD2. It is specifically involved in repair of ICL-induced DNA breaks by being required for efficient homologous recombination, probably in the resolution of homologous recombination intermediates. MTMR15 has some isoforms with MW 114 kDa and 60 kDa. The MW will be migrated to 120-140 kDa and 63-68 kDa with modification.