MTMR4 Polyklonaler Antikörper

MTMR4 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 13488-1-AP

Synonyme

EC:3.1.3.48, FYVE domain-containing dual specificity protein phosphatase 2, FYVE DSP2, FYVE-DSP2, KIAA0647

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  WB analysis using 13488-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13488-1-AP (MTMR4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of Hepatocellular carcinoma using 13488-1-AP

  Immunohistochemical analysis of paraffin-embedded hepatocellular carcinoma tissue slide using 13488-1-AP (MTMR4 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of Hepatocellular carcinoma using 13488-1-AP

  Immunohistochemical analysis of paraffin-embedded hepatocellular carcinoma tissue slide using 13488-1-AP (MTMR4 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat testis using 13488-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 13488-1-AP (MTMR4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat testis using 13488-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 13488-1-AP (MTMR4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 13488-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MTMR4 antibody (13488-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: hepatocellular carcinoma tissue, Rattenhodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

13488-1-AP bindet in WB, IHC, IF/ICC, ELISA MTMR4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MTMR4 fusion protein Ag4310
• Vollständiger Name: myotubularin related protein 4
• Berechnetes Molekulargewicht: 1195 aa, 133 kDa
• Beobachtetes Molekulargewicht: 133 kDa
• GenBank-Zugangsnummer: BC035609
• Gene symbol: MTMR4
• Gene ID (NCBI): 9110
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Myotubularin-related protein-4 (MTMR4), also named FYVE-DSP2, is a member of the myotubularin family of phosphoinositide phosphatases that selectively degrade the 3-position phosphate from the inositol ring of PtdIns(3)P and phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2) (PMID: 31543504). MTMR4 knockdown markedly suppressed the motility, fusion, and fission of PI(3)P- enriched structures, resulting in decreases in late endosomes, autophagosomes, and lysosomes, and enlargement of PI(3)P- enriched early and late endosomes(PMID: 29962048).

Publikationen

Species	Application	Title
mouse	WB	J Biol Chem; PtdIns3P phosphatases MTMR3 and MTMR4 negatively regulate innate immune responses to DNA through modulating STING trafficking.; Authors - Dyaningtyas Dewi Pamungkas Putri
human	WB	Cell Rep; RAF1 kinase contributes to autophagic lysosome reformation; Authors - Stefanie Toifl