MTMR4 Rekombinanter Antikörper

• Featured Product
• KD/KO Validated

MTMR4 Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241304E11

Kat-Nr. : 84328-1-PBS

Synonyme

KIAA0647, FYVE-DSP2, FYVE DSP2, FYVE domain-containing dual specificity protein phosphatase 2, EC:3.1.3.48

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  WB analysis using 84328-1-RR (same clone as 84328-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84328-1-RR (MTMR4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84328-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 84328-1-RR (same clone as 84328-1-PBS)

  WB result of MTMR4 antibody (84328-1-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MTMR4 transfected Jurkat cells. This data was developed using the same antibody clone with 84328-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 84328-1-RR (same clone as 84328-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MTMR4 antibody (84328-1-RR, Clone: 241304E11 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84328-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84328-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84328-1-RR against Human MTMR4 were performed. The affinity constant is 2.06 nM.

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Geprüfte Anwendungen

Produktinformation

84328-1-PBS bindet in WB, IF/ICC, Indirect ELISA MTMR4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: MTMR4 fusion protein Ag4310
• Vollständiger Name: myotubularin related protein 4
• Berechnetes Molekulargewicht: 1195 aa, 133 kDa
• Beobachtetes Molekulargewicht: 160-170 kDa
• GenBank-Zugangsnummer: BC035609
• Gene symbol: MTMR4
• Gene ID (NCBI): 9110
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Myotubularin-related protein-4 (MTMR4), also named FYVE-DSP2, is a member of the myotubularin family of phosphoinositide phosphatases that selectively degrade the 3-position phosphate from the inositol ring of PtdIns(3)P and phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2) (PMID: 31543504). MTMR4 knockdown markedly suppressed the motility, fusion, and fission of PI(3)P- enriched structures, resulting in decreases in late endosomes, autophagosomes, and lysosomes, and enlargement of PI(3)P- enriched early and late endosomes(PMID: 29962048). MTMR4 is an intracellular protein with a calculated molecular mass of 133 kDa, and detected molecular weight in the papers is about 160-170 kDa, which may be due to post-translational modification (PMID: 19125695, PMID: 20736309).