MTMR9 Rekombinanter Antikörper

MTMR9 Rekombinant Antikörper für WB, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

240167G7

Kat-Nr. : 83694-4-PBS

Synonyme

240167G7, C8orf9, Inactive phosphatidylinositol 3-phosphatase 9, LIP STYX, MTMR8

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  WB analysis using 83694-4-RR (same clone as 83694-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83694-4-RR (MTMR9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83694-4-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 83694-4-RR (same clone as 83694-4-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using MTMR9 antibody (83694-4-RR, Clone: 240167G7 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83694-4-PBS in a different storage buffer formulation.

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  FC experiment of A549 using 83694-4-RR (same clone as 83694-4-PBS)

  1x10^6 A549 cells were intracellularly stained with 0.25 ug MTMR9 Recombinant antibody (83694-4-RR, Clone:240167G7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83694-4-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 83694-4-RR (same clone as 83694-4-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug MTMR9 Recombinant antibody (83694-4-RR, Clone:240167G7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83694-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83694-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83694-4-RR against Human MTMR9 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

83694-4-PBS bindet in WB, IF/ICC, FC (Intra), ELISA MTMR9 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: MTMR9 fusion protein Ag34134
• Vollständiger Name: myotubularin related protein 9
• Berechnetes Molekulargewicht: 63 kDa
• Beobachtetes Molekulargewicht: ~60 kDa
• GenBank-Zugangsnummer: BC022003
• Gene symbol: MTMR9
• Gene ID (NCBI): 66036
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MTMR9 (Myotubularin-related protein 9) acts as an adapter for myotubularin-related phosphatases (PMID: 19038970; 22647598). MTMR9 can increase lipid phosphatase MTMR6 catalytic activity, specifically towards phosphatidylinositol 3,5-bisphosphate and MTMR6 binding affinity for phosphorylated phosphatidylinositols (PMID: 19038970; 22647598). It can also increase MTMR8 catalytic activity towards phosphatidylinositol 3-phosphate and stabilize MTMR8 protein levels (PMID: 22647598).