MTO1 Polyklonaler Antikörper

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• KD/KO Validated

MTO1 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(11)

Kat-Nr. : 15650-1-AP

Synonyme

Protein MTO1 homolog, mitochondrial, CGI-02, CGI 02

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  WB analysis using 15650-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 15650-1-AP (MTO1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 15650-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 15650-1-AP (MTO1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of MCF-7 using 15650-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 15650-1-AP (MTO1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, humanes Lebergewebe
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 9 publications below
• CoIP: See 1 publications below

Produktinformation

15650-1-AP bindet in WB, IF/ICC, CoIP, ELISA MTO1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MTO1 fusion protein Ag8192
• Vollständiger Name: mitochondrial translation optimization 1 homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 717 aa, 80 kDa
• Beobachtetes Molekulargewicht: 70-75 kDa
• GenBank-Zugangsnummer: BC005808
• Gene symbol: MTO1
• Gene ID (NCBI): 25821
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Nucleic Acids Res; Restoration of mitochondrial function through activation of hypomodified tRNAs with pathogenic mutations associated with mitochondrial diseases; Authors - Ena Tomoda
human	WB	Nat Commun; RNA modification landscape of the human mitochondrial tRNALys regulates protein synthesis.; Authors - Uwe Richter
human	CoIP	Nucleic Acids Res; The human tRNA taurine modification enzyme GTPBP3 is an active GTPase linked to mitochondrial diseases.; Authors - Gui-Xin Peng
human	WB	J Hepatol; Severe respiratory complex III defect prevents liver adaptation to prolonged fasting.; Authors - Laura S Kremer
human	WB	Am J Hum Genet; Mutations of the mitochondrial-tRNA modifier MTO1 cause hypertrophic cardiomyopathy and lactic acidosis.; Authors - Ghezzi Daniele D
human	WB	Hum Mol Genet; Altered 2-thiouridylation impairs mitochondrial translation in reversible infantile respiratory chain deficiency.; Authors - Boczonadi Veronika V