mTOR Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

mTOR Monoklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte und mehr (3)

Anwendung

WB, PLA, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

249

CloneNo.

1G11A3

Rezensionen (3)

Kat-Nr. : 66888-1-Ig

Synonyme

FLJ44809, FRAP, FRAP1, FRAP2, Mammalian target of rapamycin, MTOR, RAFT1, Rapamycin target protein 1, RAPT1

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Galerie der Validierungsdaten

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  WB analysis using 66888-1-Ig

  Various lysates (25 μg/lane) were subjected to SDS PAGE followed by western blot with 66888-1-Ig (MTOR antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66888-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66888-1-Ig (mTOR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66888-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66888-1-Ig (mTOR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66888-1-Ig

  WB result of mTOR antibody (66888-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-mTOR transfected HeLa cells.

• 

  WB analysis of HeLa using 66888-1-Ig

  Non-treated and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 67778-1-Ig (Phospho-mTOR (Ser2448) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with RICTOR antibody (66867-2-Ig) and mTOR antibody (66888-1-Ig) subsequently.

• 

  WB analysis of HEK-293 using 66888-1-Ig

  Non-treated and Rapamycin treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 67778-1-Ig (Phospho-mTOR (Ser2448) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with RICTOR antibody (66867-2-Ig) and mTOR antibody (66888-1-Ig) subsequently.

• 

  WB analysis of HEK-293 using 66888-1-Ig

  Non-treated and Calyculin A treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 67778-1-Ig (Phospho-mTOR (Ser2448) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with RICTOR antibody (66867-2-Ig) and mTOR antibody (66888-1-Ig) subsequently.

• 

  WB analysis of ROS1728 using 66888-1-Ig

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 66888-1-Ig (mTOR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 66888-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66888-1-Ig (mTOR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 66888-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66888-1-Ig (mTOR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 66888-1-Ig

  IP result of anti-mTOR (IP:66888-1-Ig, 5ug; Detection:66888-1-Ig 1:20000) with HeLa cells lysate 1600 ug.

• 

  IP experiment of HeLa using 66888-1-Ig

  IP result of anti-mTOR (IP:66888-1-Ig, 5ug; Detection:66888-1-Ig 1:20000) with HeLa cells lysate 1600 ug.

• 

  IHC staining of human colon cancer using 66888-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66888-1-Ig (MTOR antibody) at dilution of 1:2500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66888-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66888-1-Ig (MTOR antibody) at dilution of 1:2500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66888-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66888-1-Ig (MTOR antibody) at dilution of 1:2500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66888-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66888-1-Ig (MTOR antibody) at dilution of 1:2500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66888-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66888-1-Ig (MTOR antibody) at dilution of 1:2500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66888-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66888-1-Ig (MTOR antibody) at dilution of 1:2500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 66888-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using mTOR antibody (66888-1-Ig, Clone: 1G11A3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HepG2 using 66888-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using mTOR antibody (66888-1-Ig, Clone: 1G11A3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HepG2 using 66888-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using mTOR antibody (66888-1-Ig, Clone: 1G11A3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HepG2 using 66888-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using mTOR antibody (66888-1-Ig, Clone: 1G11A3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: LNCaP-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, Maushirngewebe, NCI-H1299-Zellen, NIH/3T3-Zellen, Rattenhirngewebe, ROS1728-Zellen, THP-1-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Leberkarzinomgewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 241 publications below
• IHC: See 7 publications below
• IF: See 6 publications below
• IP: See 4 publications below

Produktinformation

66888-1-Ig bindet in WB, PLA, IP, IHC, IF, ELISA mTOR und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, hamster, Hausschwein, Maus, Ratte, Ziege
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: mTOR fusion protein Ag28395
• Vollständiger Name: FK506 binding protein 12-rapamycin associated protein 1
• Berechnetes Molekulargewicht: 289 kDa
• Beobachtetes Molekulargewicht: 250-289 kDa
• GenBank-Zugangsnummer: NM_004958
• Gene symbol: FRAP1
• Gene ID (NCBI): 2475
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MTOR, also named as FRAP1, FRAP, FRAP2 and RAPT1, belongs to the PI3/PI4-kinase family. MTOR is a Ser/Thr protein kinase that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth. MTOR is kinase subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. MTOR has a calculated molecular mass of 289 kDa, and always can be detected at about 250 kDa due to some modifications (PMID: 14578359).

Publikationen

Species	Application	Title
human	WB	Gastroenterology; Nucleolar HEATR1 upregulated by mTORC1 signaling promotes hepatocellular carcinoma growth by dominating ribosome biogenesis and proteome homeostasis; Authors - Xiao-Mei Yang
human	WB,IP	Mol Cell; Lysosomal cyst(e)ine storage potentiates tolerance to oxidative stress in cancer cells; Authors - Lixin He
human	WB	Mol Cancer; circEXOC6B interacting with RRAGB, an mTORC1 activator, inhibits the progression of colorectal cancer by antagonizing the HIF1A-RRAGB-mTORC1 positive feedback loop.; Authors - Xiaomin Li
human	WB	Autophagy; Periplocin suppresses the growth of colorectal cancer cells by triggering LGALS3 (galectin 3)-mediated lysophagy; Authors - Kui Wang
mouse	PLA	Nat Commun; Location-specific inhibition of Akt reveals regulation of mTORC1 activity in the nucleus.; Authors - Xin Zhou
human	WB	J Clin Invest; Up-regulated YB-1 protein promotes glioblastoma growth through an YB-1/CCT4/mLST8/mTOR pathway.; Authors - Jin-Zhu Wang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Sun (Verified Customer) (01-05-2023)

strong band showed up around 200kDa region in HEK293T cells.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T

FH

Macarena (Verified Customer) (10-07-2022)

excellent band. Very concentrated.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: cell lines

FH

Rachel (Verified Customer) (05-24-2021)

Lovely antibody, very clean good siganl Cell lysates were run on JESS Simple Western

• Applications: Western Blot
• Primary Antibody Dilution: 1/50
• Cell Tissue Type: PC3 cells

mTOR Antibody Western Blot validation (1/50 dilution) in PC3 cells (Cat no:66888-1-Ig)