MTR Polyklonaler Antikörper

MTR Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(15)

Kat-Nr. : 25896-1-AP

Synonyme

Methionine synthase, 5-methyltetrahydrofolate--homocysteine methyltransferase, cblG, Cobalamin-dependent methionine synthase, EC:2.1.1.13

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  WB analysis using 25896-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25896-1-AP (MTR antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 25896-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 25896-1-AP (MTR Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse pancreas using 25896-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 25896-1-AP (MTR Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human cervical cancer using 25896-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 25896-1-AP (MTR antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 25896-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 25896-1-AP (MTR antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 25896-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 25896-1-AP (MTR Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 25896-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 25896-1-AP (MTR Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human pancreas using 25896-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 25896-1-AP (MTR Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 25896-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 25896-1-AP (MTR Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of A375 using 25896-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A375 cells using 25896-1-AP (MTR antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A375-Zellen, HEK-293-Zellen, HepG2-Zellen, L02-Zellen, Maus-Pankreasgewebe
• Erfolgreiche Detektion in IHC: humanes Zervixkarzinomgewebe, humanes Herzgewebe, humanes Pankreasgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A375-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 13 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

25896-1-AP bindet in WB, IHC, IF/ICC, ELISA MTR und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MTR fusion protein Ag23156
• Vollständiger Name: 5-methyltetrahydrofolate-homocysteine methyltransferase
• Beobachtetes Molekulargewicht: 140 kDa
• GenBank-Zugangsnummer: BC130616
• Gene symbol: MTR
• Gene ID (NCBI): 4548
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MTR (Methionine synthase), one of two B12-dependent mammalian enzymes, catalyzes a methyl transfer from methyltetrahydrofolate to homocysteine, generating methionine and tetrahydrofolate (PMID: 9587027, 9013615). Methionine synthase levels in mammalian cells are influenced by folate status, and the ability of cells to grow in the absence of methionine is partly rate-limited by synthase activity levels. MTR loss depletes cells and tumors of reduced folate species (PMID: 34799699)

Publikationen

Species	Application	Title
human		Cell Metab; Transsulfuration Activity Can Support Cell Growth upon Extracellular Cysteine Limitation.; Authors - Jiajun Zhu
human	WB	Mol Cell; mTORC1 stimulates cell growth through SAM synthesis and m6A mRNA-dependent control of protein synthesis.; Authors - Elodie Villa
human	WB	J Pineal Res; Melatonin modulates metabolic remodeling in HNSCC by suppressing MTHFD1L-formate axis.; Authors - Li Cui
mouse	WB	Clin Epigenetics; A transgenic mice model of retinopathy of cblG-type inherited disorder of one-carbon metabolism highlights epigenome-wide alterations related to cone photoreceptor cells development and retinal metabolism; Authors - Karim Matmat
mouse	WB	Nutrients; Mild Choline Deficiency and MTHFD1 Synthetase Deficiency Interact to Increase Incidence of Developmental Delays and Defects in Mice.; Authors - Karen E Christensen
human	WB	iScience; Linkage of methionine addiction, histone lysine hypermethylation, and malignancy.; Authors - Jun Yamamoto