MTSS1 Polyklonaler Antikörper

MTSS1 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 13995-1-AP

Synonyme

KIAA0429, metastasis suppressor 1, Metastasis suppressor YGL 1, MIM, MIMA, MIMB, Missing in metastasis protein, MTSS1

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Galerie der Validierungsdaten

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  WB analysis using 13995-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13995-1-AP (MTSS1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 13995-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13995-1-AP (MTSS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 13995-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13995-1-AP (MTSS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 13995-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13995-1-AP (MTSS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 13995-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13995-1-AP (MTSS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 13995-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using MTSS1 antibody (13995-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, Mausnierengewebe, MCF-7-Zellen, NIH/3T3-Zellen, Rattennierengewebe
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IF: See 1 publications below

Produktinformation

13995-1-AP bindet in WB, IHC, IF, ELISA MTSS1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MTSS1 fusion protein Ag5080
• Vollständiger Name: metastasis suppressor 1
• Berechnetes Molekulargewicht: 82 kDa
• Beobachtetes Molekulargewicht: 82 kDa
• GenBank-Zugangsnummer: BC023998
• Gene symbol: MTSS1
• Gene ID (NCBI): 9788
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MTSS1 (metastasis suppressor 1), also known as MIM (missing-in-metastasis), MIM-B, BEG4 (Basal cell carcinoma-enriched gene 4) or KIAA0429, is one of the defining members of the I-BAR family of negative membrane curvature-sensing proteins first identified as being deleted in metastatic bladder cancer. MTSS1 over-expression is associated with enhanced cell migration, resulting in tumorigenesis, invasion and metastasis (PMID: 27230279). It is reported that the expression of MTSS1 is associated with poor prognosis in various cancers,such as bladder, gastric, breast and so on (PMID: 32052679).

Publikationen

Species	Application	Title
human	WB,IF	Cytoskeleton (Hoboken); Metastasis suppressor 1 interacts with α-actinin 4 to affect its localization and regulate formation of membrane ruffling.; Authors - Lijun Liang ; KD Validated