MTSS1L Polyklonaler Antikörper

MTSS1L Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 27832-1-AP

Synonyme

Protein MTSS 2, MTSS2, MTSS1-like protein, Actin-bundling with BAIAP2 homology protein 1, ABBA-1

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  WB analysis using 27832-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27832-1-AP (MTSS1L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 27832-1-AP

  IP result of anti-MTSS1L (IP:27832-1-AP, 4ug; Detection:27832-1-AP 1:300) with Jurkat cells lysate 4000 ug.

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  IHC staining of mouse brain using 27832-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27832-1-AP (MTSS1L antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 27832-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27832-1-AP (MTSS1L antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 27832-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using MTSS1L antibody (27832-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of MCF-7 using 27832-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 27832-1-AP (MTSS1L antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen, HeLa-Zellen, Jurkat-Zellen, Maushirngewebe, NIH/3T3-Zellen, Rattenhirngewebe, U-251-Zellen
• Erfolgreiche IP: Jurkat-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

27832-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA MTSS1L und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MTSS1L fusion protein Ag27216
• Vollständiger Name: metastasis suppressor 1-like
• Beobachtetes Molekulargewicht: 68-70 kDa
• GenBank-Zugangsnummer: BC002770
• Gene symbol: MTSS1L
• Gene ID (NCBI): 92154
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MTSS2, also known as MTSS1L, contains a conserved N-terminal domain, called an IRSP53 /MIM homology domain (IMD) or inverse BAR domain, that is involved in plasma membrane dynamics (PMID: 20875796). MTSS2 potentiated PDGF-mediated formation of membrane ruffles and lamellipodia in fibroblasts, acting via RAC1 activation (PMID:14752106). MTSS2 is implicated in intellectual developmental disorders with ocular anomalies and distinctive facial features(PMID: 36067766).

Publikationen

Species	Application	Title
human	WB	Cell Discov; m6Am methyltransferase PCIF1 is essential for aggressiveness of gastric cancer cells by inhibiting TM9SF1 mRNA translation.; Authors - Wei Zhuo
human	WB	Acta Biochim Biophys Sin (Shanghai); MicroRNA-23a promotes colorectal cancer cell migration and proliferation by targeting at MARK1.; Authors - Xiaoli Tang