MTUS1 Polyklonaler Antikörper

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• KD/KO Validated

MTUS1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13436-1-PBS

Synonyme

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  WB analysis using 13436-1-AP (same clone as 13436-1-PBS)

  Jurkat lysates were subjected to SDS PAGE followed by western blot with 13436-1-AP (MTUS1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13436-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 13436-1-AP (same clone as 13436-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13436-1-AP (MTUS1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13436-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 13436-1-AP (same clone as 13436-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 13436-1-AP (MTUS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13436-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 13436-1-AP (same clone as 13436-1-PBS)

  IP result of anti-MTUS1 (IP:13436-1-AP, 4ug; Detection:13436-1-AP 1:500) with mouse brain tissue lysate 6000ug. This data was developed using the same antibody clone with 13436-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 13436-1-AP (same clone as 13436-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13436-1-AP (MTUS1 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13436-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 13436-1-AP (same clone as 13436-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13436-1-AP (MTUS1 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13436-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human thyroid cancer using 13436-1-AP (same clone as 13436-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 13436-1-AP (MTUS1 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13436-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human thyroid cancer using 13436-1-AP (same clone as 13436-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 13436-1-AP (MTUS1 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13436-1-PBS in a different storage buffer formulation.

• 

  IF Staining of PC-3 using 13436-1-AP (same clone as 13436-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol) fixed PC-3 cells using 13436-1-AP (MTUS1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13436-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

13436-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA MTUS1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MTUS1 fusion protein Ag4242
• Vollständiger Name: mitochondrial tumor suppressor 1
• Berechnetes Molekulargewicht: 51 kDa, 141 kDa
• Beobachtetes Molekulargewicht: 40-48 kDa, 80 kDa, 110-120 kDa, 270-280 kDa
• GenBank-Zugangsnummer: BC033842
• Gene symbol: MTUS1
• Gene ID (NCBI): 57509
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MTUS1, also named as ATBP, ATIP, GK1, KIAA1288 and MTSG1, Belongs to the MTUS1 family. It cooperates with AGTR2 to inhibit ERK2 activation and cell proliferation. MTUS1 may be required for AGTR2 cell surface expression. Together with PTPN6, induces UBE2V2 expression upon angiotensin-II stimulation. Isoform 1 inhibits breast cancer cell proliferation, delays the progression of mitosis by prolonging metaphase and reduces tumor growth. MTUS1 up-regulation during cellular transition from proliferation to quiescence and differentiation. It is a potential tumor suppressor gene located at chromosome 8p21.3.22, near marker D8S254. According to the functional data and intracellular localization, MTUS1 also named as mitochondrial tumor suppressor gene 1 (MTSG1). One main feature common to all ATIP members is the presence of a large C-terminal coiled-coil domain that allows homo- and hetero-dimerization of these proteins. (PMID:12692079, 15123706). The antibody can recognize all the isoforms expect isoform 5(85-90 kDa). The antibody tested HomoDimer isoforms (80kd/110-120/280kd) in Jurkat cell.