MUC16/CA125 Polyklonaler Antikörper

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• KD/KO Validated

MUC16/CA125 Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 20077-1-PBS

Synonyme

MUC16, MUC16,CA125, MUC-16, MUC 16, CA125

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  WB analysis of HeLa using 20077-1-AP (same clone as 20077-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 20077-1-AP (MUC16,CA125 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20077-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 20077-1-AP (same clone as 20077-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 20077-1-AP (MUC16,CA125 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20077-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HeLa using 20077-1-AP (same clone as 20077-1-PBS)

  IP result of anti-MUC16,CA125 (IP:20077-1-AP, 4ug; Detection:20077-1-AP 1:600) with HeLa cells lysate 1200ug. This data was developed using the same antibody clone with 20077-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 20077-1-AP (same clone as 20077-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 20077-1-AP (MUC16,CA125 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20077-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 20077-1-AP (same clone as 20077-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 20077-1-AP (MUC16,CA125 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20077-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

20077-1-PBS bindet in WB, IHC, IP, Indirect ELISA MUC16/CA125 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: mucin 16, cell surface associated
• Berechnetes Molekulargewicht: 1519 kDa
• Beobachtetes Molekulargewicht: 90-100 kDa
• GenBank-Zugangsnummer: NM_024690
• Gene symbol: MUC16
• Gene ID (NCBI): 94025
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MUC16, also named as CA125, is thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. It is a serum marker that is used routinely in gynecologic practice to monitor patients with ovarian cancer. Most biochemical studies have concluded that MUC16 is a high molecular mass glycoprotein, although estimates of its size range from 200 to 2000 kDa with smaller "subunits" being described by some investigators. The antibody recognizes the C-term of MUC16.