MUC2 Polyklonaler Antikörper

MUC2 Polyklonal Antikörper für IHC, IF/ICC, IF-P, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (4)

Anwendung

WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(107)

Rezensionen (1)

Kat-Nr. : 27675-1-AP

Synonyme

MUC 2, MUC-2, Mucin 2, SMUC

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  IHC staining of human colon using 27675-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27675-1-AP (MUC2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 27675-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27675-1-AP (MUC2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat small intestine using 27675-1-AP

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 27675-1-AP (MUC2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 27675-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 27675-1-AP (MUC2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat small intestine using 27675-1-AP

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 27675-1-AP (MUC2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse small intestine using 27675-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 27675-1-AP (MUC2 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse small intestine using 27675-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 27675-1-AP (MUC2 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of rat small intestine using 27675-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat small intestine tissue using MUC2 antibody (27675-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), smooth muscle actin specific antibody (67735-1-Ig, Clone: 1E9A11, red). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of rat small intestine using 27675-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat small intestine tissue using MUC2 antibody (27675-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), smooth muscle actin specific antibody (67735-1-Ig, Clone: 1E9A11, red). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of rat small intestine using 27675-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat small intestine tissue using MUC2 antibody (27675-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004), smooth muscle actin specific antibody (67735-1-Ig, Clone: 1E9A11, green). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human colon using 27675-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human colon tissue using MUC2 antibody (27675-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse colon using 27675-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using MUC2 antibody (27675-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of SW480 using 27675-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed SW480 cells using MUC2 antibody (27675-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 27675-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using MUC2 antibody (27675-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of MCF-7 using 27675-1-AP

  1x10^6 MCF-7 cells were intracellularly stained with 0.13 ug MUC2 Polyclonal antibody (27675-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Kolongewebe, humanes Pankreaskarzinomgewebe, Maus-Dünndarmgewebe, Ratten-Dünndarmgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: humanes Kolongewebe, Maus-Kolongewebe, Ratten-Dünndarmgewebe
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen, SW480-Zellen
• Erfolgreiche Detektion in FC (Intra): MCF-7-Zellen

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 25 publications below
• IHC: See 42 publications below
• IF: See 53 publications below

Produktinformation

27675-1-AP bindet in WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA MUC2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ente, Hausschwein, Huhn, Maus, Ratte, Stechmücke
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MUC2 fusion protein Ag25800
• Vollständiger Name: mucin 2, oligomeric mucus/gel-forming
• Berechnetes Molekulargewicht: 540 kDa
• GenBank-Zugangsnummer: M94132
• Gene symbol: MUC2
• Gene ID (NCBI): 4583
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

This gene encodes a member of the mucin protein family. Mucins are high molecular weight glycoproteins produced by many epithelial tissues. The protein encoded by this gene is secreted and forms an insoluble mucous barrier that protects the gut lumen. The protein polymerizes into a gel of which 80% is composed of oligosaccharide side chains by weight. Downregulation of this gene has been observed in patients with Crohn disease and ulcerative colitis.

Publikationen

Species	Application	Title
mouse	IF	Nat Commun; Dubosiella newyorkensis modulates immune tolerance in colitis via the L-lysine-activated AhR-IDO1-Kyn pathway; Authors - Yanan Zhang
human,mouse	IHC,IF	Acta Pharm Sin B; PIM1-HDAC2 axis modulates intestinal homeostasis through epigenetic modification; Authors - Jianming Yang
mouse	IF	EMBO J; Myeloid cells protect intestinal epithelial barrier integrity through the angiogenin/plexin-B2 axis.; Authors - Rongpan Bai
mouse	IHC,IF	Carbohydr Polym; Polygonatum cyrtonema Hua polysaccharide alleviates ulcerative colitis via gut microbiota-independent modulation of inflammatory immune response; Authors - Jin-Yang Shi
mouse	IF	Pharmacol Res; λ-carrageenan exacerbates Citrobacter rodentium-induced infectious colitis in mice by targeting gut microbiota and intestinal barrier integrity; Authors - Wei Wu
mouse	IF	NPJ Biofilms Microbiomes; Impact of structurally diverse polysaccharides on colonic mucin O-glycosylation and gut microbiota; Authors - Tong Zhao

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Balawant (Verified Customer) (08-24-2022)

This is great Antibody. it has no background

• Applications: Western Blot
• Primary Antibody Dilution: 1:1500
• Cell Tissue Type: colon tissue and colon cancer cells line