MUL1 Polyklonaler Antikörper

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• KD/KO Validated

MUL1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(12)

Kat-Nr. : 16133-1-AP

Synonyme

C1orf166, GIDE, MAPL, MUL1, MULAN, RING finger protein 218, RNF218, RP11 401M16.2

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  WB analysis of HeLa using 16133-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16133-1-AP (MUL1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 16133-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16133-1-AP (MUL1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 16133-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16133-1-AP (MUL1 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:300-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 11 publications below
• IHC: See 2 publications below
• IP: See 3 publications below
• CoIP: See 1 publications below

Produktinformation

16133-1-AP bindet in WB, IHC, IP, CoIP, ELISA MUL1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MUL1 fusion protein Ag9119
• Vollständiger Name: mitochondrial E3 ubiquitin ligase 1
• Berechnetes Molekulargewicht: 352 aa, 40 kDa
• Beobachtetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: BC010101
• Gene symbol: MUL1
• Gene ID (NCBI): 79594
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Mitochondrial ubiquitin ligase 1 (MUL1),MAPL, MULAN, or gGIDE, was identified as an E3 protein ligase by three independent groups. Work in mammalian systems shows that MUL1 has small ubiquitin-like modifier (SUMO) ligase activity, stabilizing Drp1, or ubiquitin ligase activity, degrading Mfn. MUL1 expression in mammalian cells results in smaller and more fragmented mitochondria.

Publikationen

Species	Application	Title
human	WB	J Exp Clin Cancer Res; PHB2 promotes SHIP2 ubiquitination via the E3 ligase NEDD4 to regulate AKT signaling in gastric cancer; Authors - Liang Xu
rat	WB	Oxid Med Cell Longev; Inhibition of Prostaglandin E2 Receptor EP3 Attenuates Oxidative Stress and Neuronal Apoptosis Partially by Modulating p38MAPK/FOXO3/Mul1/Mfn2 Pathway after Subarachnoid Hemorrhage in Rats; Authors - Yu Liu
mouse	WB	Life Sci; Trichostatin A inhibits skeletal muscle atrophy induced by cigarette smoke exposure in mice.; Authors - Jingjing Ding
human,mouse	WB	Eur J Pharmacol; Mitochondrial E3 ubiquitin ligase 1 promotes brain injury by disturbing mitochondrial dynamics in a rat model of ischemic stroke.; Authors - Kai-Di Ren ; KD Validated
rat	CoIP	Can J Physiol Pharmacol; Upregulation of mitochondrial E3 ubiquitin ligase 1 in rat heart contributes to ischemia/reperfusion injury.; Authors - Shi-Jing Wang
rat	WB,IP	J Ginseng Res; Ginsenoside compound K protects against cerebral ischemia/reperfusion injury via Mul1/Mfn2-mediated mitochondrial dynamics and bioenergy; Authors - Qingxia Huang