MUM1 Polyklonaler Antikörper

MUM1 Polyklonal Antikörper für IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Rezensionen (1)

Kat-Nr. : 12682-1-AP

Synonyme

EXPAND1, HSPC211, MUM 1, MUM1, Protein expandere, PWWP3A

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Galerie der Validierungsdaten

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  WB analysis using 12682-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12682-1-AP (MUM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HT-1080 using 12682-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 12682-1-AP (MUM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 12682-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 12682-1-AP (MUM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 12682-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12682-1-AP (MUM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 12682-1-AP

  IP Result of anti-MUM1 (IP:12682-1-AP, 4ug; Detection:12682-1-AP 1:300) with Jurkat cells lysate 4000ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HT-1080.Zellen, Jurkat-Zellen
• Erfolgreiche IP: Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

12682-1-AP bindet in WB, IP, ELISA MUM1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MUM1 fusion protein Ag3371
• Vollständiger Name: melanoma associated antigen (mutated) 1
• Berechnetes Molekulargewicht: 18 kDa, 79 kDa
• Beobachtetes Molekulargewicht: 70-80 kDa
• GenBank-Zugangsnummer: BC008098
• Gene symbol: MUM1
• Gene ID (NCBI): 84939
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MUM1, also named as EXPAND1, is an architectural component of the chromatin, which in response to DNA damage serves as an accessory factor to promote cell survival. It is a 53BP1-associated DNA damage-responsive factor. MUM1/EXPAND1 is different to another protein IRF4/MUM1.

Publikationen

Species	Application	Title
mouse	WB	Nature; Post-translational control of beige fat biogenesis by PRDM16 stabilization; Authors - Qiang Wang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Aurelie (Verified Customer) (09-01-2019)

Lysats (20µg) from Mouse cortex were loaded in a 8% SDS-PAGE and immunoblotted using anti-MUM1 antibody ( 1/1000) over-night at 4°C.

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Mouse Cortex

MUM1 Antibody Western Blot, validation (1/1000 dilution) in Mouse Cortex (Cat no:12682-1-AP)