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MUM1/IRF4 Polyklonaler Antikörper

MUM1/IRF4 Polyklonal Antikörper für FC(Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC(Intra)

Konjugation

CoraLite®555 Fluorescent Dye

Kat-Nr. : CL555-11247

Synonyme

interferon regulatory factor 4, IRF 4, IRF4, LSIRF, Multiple myeloma oncogene 1, MUM1, MUM1/IRF4, NF EM5

Galerie der Validierungsdaten

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  • Flow cytometry (FC) experiment of Ramos cells using CoraLite®555-conjugated MUM1/IRF4 Polyclonal antib (CL555-11247)

    FC experiment of Ramos using CL555-11247

    1X10^6 Ramos cells were intracellularly stained with 0.8 ug CoraLite®555 Anti-Human MUM1/IRF4 (CL555-11247) (red), or 0.8 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Geprüfte Anwendungen

Erfolgreiche Detektion in FCRamos-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Durchflusszytometrie (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL555-11247 bindet in FC(Intra) MUM1/IRF4 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen MUM1/IRF4 fusion protein Ag1762
Vollständiger Name IRF 4
Berechnetes Molekulargewicht 52 kDa
Beobachtetes Molekulargewicht 52 kDa
GenBank-ZugangsnummerBC015752
Gene symbol IRF4
Gene ID (NCBI) 3662
Konjugation CoraLite®555 Fluorescent Dye
Excitation/Emission maxima wavelengths557 nm / 570nm
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IRF4 is a member of the IRF family of transcription factors, expressed in most cell types of the immune system. IRF4 is a transcription factor essential for the development of T helper-2 (Th2) cells, IL17-producing Th17 cells, and IL9-producing Th9 cells, as well as dendritic cell (DC). It binds to and activates the ISRE of the MHC class I promoter, and may have a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells.

Protokolle

Produktspezifische Protokolle
FC protocol for CL555 MUM1/IRF4 antibody CL555-11247Protokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen