MUS81 Polyklonaler Antikörper

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• KD/KO Validated

MUS81 Polyklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 11018-1-AP

Synonyme

MUS81

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Galerie der Validierungsdaten

• 

  WB analysis using 11018-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11018-1-AP (MUS81 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 11018-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 11018-1-AP (MUS81 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of MCF-7 using 11018-1-AP

  Immunofluorescent analysis of MCF-7 cells, using MUS81 antibody 11018-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IF: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Produktinformation

11018-1-AP bindet in WB, IHC, IF, ELISA MUS81 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MUS81 fusion protein Ag1476
• Vollständiger Name: MUS81 endonuclease homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 61 kDa
• Beobachtetes Molekulargewicht: 52-72 kDa
• GenBank-Zugangsnummer: BC009999
• Gene symbol: MUS81
• Gene ID (NCBI): 80198
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The Crossover junction endonuclease MUS81 has associated endonuclease activity against structure-specific oligonucleotide substrates, including synthetic Holliday junctions(PMID:11741546). It interacts with EME1 and EME2 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. The open reading frame of human MUS81 predicts a translation product of 551 amino acids with a molecular mass of 59 kD. There are some reports showed that the enzyme can express a 72 kDa protein in human tissues(PMID:12724407).

Publikationen

Species	Application	Title
human	WB,IHC	Cell; EMSY inhibits homologous recombination repair and the interferon response, promoting lung cancer immune evasion.; Authors - Antonio Marzio ; KD Validated
	WB	Elife; 53BP1 and BRCA1 control pathway choice for stalled replication restart.; Authors - Yixi Xu