Methylmalonyl Coenzyme A mutase/MUT Polyklonaler Antikörper

Methylmalonyl Coenzyme A mutase/MUT Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 17034-1-PBS

Synonyme

MUT, EC:5.4.99.2, MCM, Methylmalonyl CoA isomerase, Methylmalonyl Coenzyme A mutase

---

• 

  WB analysis using 17034-1-AP (same clone as 17034-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 17034-1-AP (Methylmalonyl Coenzyme A mutase/MUT antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17034-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HepG2 using 17034-1-AP (same clone as 17034-1-PBS)

  IP result of anti-Methylmalonyl Coenzyme A mutase/MUT (IP:17034-1-AP, 4ug; Detection:17034-1-AP 1:500) with HepG2 cells lysate 1800ug. This data was developed using the same antibody clone with 17034-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 17034-1-AP (same clone as 17034-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17034-1-AP (Methylmalonyl Coenzyme A mutase/MUT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17034-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 17034-1-AP (same clone as 17034-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17034-1-AP (Methylmalonyl Coenzyme A mutase/MUT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17034-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 17034-1-AP (same clone as 17034-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 17034-1-AP (Methylmalonyl Coenzyme A mutase/MUT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17034-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 17034-1-AP (same clone as 17034-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 17034-1-AP (Methylmalonyl Coenzyme A mutase/MUT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17034-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

17034-1-PBS bindet in WB, IHC, IP, Indirect ELISA Methylmalonyl Coenzyme A mutase/MUT und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Methylmalonyl Coenzyme A mutase/MUT fusion protein Ag10523
• Vollständiger Name: methylmalonyl Coenzyme A mutase
• Berechnetes Molekulargewicht: 750 aa, 83 kDa
• Beobachtetes Molekulargewicht: 78 kDa
• GenBank-Zugangsnummer: BC016282
• Gene symbol: MUT
• Gene ID (NCBI): 4594
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Methylmalonyl Coenzyme A mutase (MUT) is an enzyme that plays a crucial role in the metabolism of certain amino acids and fatty acids (PMID: 15647267). Mutations in the MUT gene can lead to methylmalonic acidemia, a metabolic disorder characterized by the accumulation of toxic compounds such as methylmalonyl-CoA and propionyl-CoA (PMID: 30428564), resulting in life-threatening metabolic acidosis, respiratory distress, neurological impairment, hyperammonemia, and ketosis (PMID: 32679819). MUT is essential for maintaining normal metabolic processes and its dysfunction can have significant health implications, highlighting its importance in both basic metabolism and clinical medicine (PMID: 23041189).