Methylmalonyl Coenzyme A mutase/MUT Rekombinanter Antikörper

Methylmalonyl Coenzyme A mutase/MUT Rekombinant Antikörper für IHC, IF/ICC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IHC, IF/ICC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242198A3

Kat-Nr. : 84879-6-PBS

Synonyme

MUT, EC:5.4.99.2, MCM, Methylmalonyl CoA isomerase, Methylmalonyl Coenzyme A mutase

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  IHC staining of Human Hepatocellular cancer using 84879-6-RR (same clone as 84879-6-PBS)

  Immunohistochemical analysis of paraffin-embedded Human Hepatocellular cancer slide using 84879-6-RR (Methylmalonyl Coenzyme A mutase/MUT antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84879-6-PBS in a different storage buffer formulation.

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  IHC staining of Human Hepatocellular cancer using 84879-6-RR (same clone as 84879-6-PBS)

  Immunohistochemical analysis of paraffin-embedded Human Hepatocellular cancer slide using 84879-6-RR (Methylmalonyl Coenzyme A mutase/MUT antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84879-6-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 84879-6-RR (same clone as 84879-6-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Methylmalonyl Coenzyme A mutase/MUTantibody (84879-6-RR, Clone: 242198A3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84879-6-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 84879-6-RR (same clone as 84879-6-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Methylmalonyl Coenzyme A mutase/MUT Recombinant Antibody (84879-6-RR, Clone:242198A3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84879-6-PBS in a different storage buffer formulation.

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  FC experiment of A431 using 84879-6-RR (same clone as 84879-6-PBS)

  1x10^6 A431 cells were intracellularly stained with 0.25 ug Methylmalonyl Coenzyme A mutase/MUT Recombinant Antibody (84879-6-RR, Clone:242198A3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84879-6-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

84879-6-PBS bindet in IHC, IF/ICC, FC (Intra), Indirect ELISA Methylmalonyl Coenzyme A mutase/MUT und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Methylmalonyl Coenzyme A mutase/MUT fusion protein Ag10523
• Vollständiger Name: methylmalonyl Coenzyme A mutase
• Berechnetes Molekulargewicht: 750 aa, 83 kDa
• Beobachtetes Molekulargewicht: 78 kDa
• GenBank-Zugangsnummer: BC016282
• Gene symbol: MUT
• Gene ID (NCBI): 4594
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Methylmalonyl Coenzyme A mutase (MUT) is an enzyme that plays a crucial role in the metabolism of certain amino acids and fatty acids. Mutations in the MUT gene can lead to methylmalonic acidemia, a metabolic disorder characterized by the accumulation of toxic compounds such as methylmalonyl-CoA and propionyl-CoA. This condition can cause severe health issues including developmental delays, metabolic acidosis, and neurological problems. MUT is essential for maintaining normal metabolic processes and its dysfunction can have significant health implications, highlighting its importance in both basic metabolism and clinical medicine.