MUTYH Polyklonaler Antikörper

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• KD/KO Validated

MUTYH Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 19650-1-AP

Synonyme

hMYH, MutY homolog, mutY homolog (E. coli), MUTYH, MYH

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  WB analysis of HeLa using 19650-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19650-1-AP (MUTYH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 19650-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 19650-1-AP (MUTYH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 19650-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 19650-1-AP (MUTYH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 19650-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 19650-1-AP (MUTYH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 19650-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 19650-1-AP (MUTYH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 19650-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 19650-1-AP (MUTYH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 19650-1-AP

  IP result of anti-MUTYH (IP:19650-1-AP, 4ug; Detection:19650-1-AP 1:500) with HeLa cells lysate 3440ug.

• 

  IHC staining of human kidney using 19650-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 19650-1-AP (MUTYH Antibody) at dilution of 1:50.

• 

  IHC staining of mouse liver using 19650-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 19650-1-AP (MUTYH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 19650-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 19650-1-AP (MUTYH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 19650-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 19650-1-AP (MUTYH Antibody) at dilution of 1:50.

• 

  IHC staining of human colon cancer using 19650-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 19650-1-AP (MUTYH Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 19650-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 19650-1-AP (MUTYH Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, COLO 320-Zellen, Jurkat-Zellen, K-562-Zellen, Maus-Thymusgewebe, Raji-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: Mauslebergewebe, humanes Kolonkarzinomgewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below

Produktinformation

19650-1-AP bindet in WB, IP, IHC, ELISA MUTYH und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MUTYH fusion protein Ag7526
• Vollständiger Name: mutY homolog (E. coli)
• Berechnetes Molekulargewicht: 60 kDa
• Beobachtetes Molekulargewicht: 53-64 kDa
• GenBank-Zugangsnummer: BC003178
• Gene symbol: MUTYH
• Gene ID (NCBI): 4595
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MUTYH(MutY homolog) is also named as MYH and belongs to the Nth/MutY family. The gene enconde an A/G-specific adenine DNA glycosylase that it is involved in oxidative DNA damage repair and initiates repair of A*oxoG to C*G by removing the inappropriately paired adenine base from the DNA backbone. Possesses both adenine and 2-OH-A DNA glycosylase activities(PMID:10684930). Defects in MUTYH are a cause of familial adenomatous polyposis type 2 (FAP2) and gastric cancer (GASC). It has 6 isoforms produced by alternative splicing with the molecular weight between 57 kDa and 60 kDa.

Publikationen

Species	Application	Title
mouse	WB	Oxid Med Cell Longev; MUTYH Actively Contributes to Microglial Activation and Impaired Neurogenesis in the Pathogenesis of Alzheimer's Disease; Authors - Yuri Mizuno
human	WB	Oncol Rep; Downregulation of MUTYH contributes to cisplatin‑resistance of esophageal squamous cell carcinoma cells by promoting Twist‑mediated EMT.; Authors - Yanxia Guo ; KD Validated
human	WB	Cell Death Dis; Potent and specific MTH1 inhibitors targeting gastric cancer.; Authors - Wenjuan Zhou