MVD Polyklonaler Antikörper

MVD Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Rezensionen (1)

Kat-Nr. : 15331-1-AP

Synonyme

FP17780, MDDase, MPD, MVD

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Galerie der Validierungsdaten

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  WB analysis of HepG2 using 15331-1-PBS

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15331-1-AP (MVD antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis using 15331-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15331-1-AP (MVD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat liver using 15331-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 15331-1-AP (MVD Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 15331-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15331-1-AP (MVD antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 15331-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15331-1-AP (MVD Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 15331-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15331-1-AP (MVD Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 15331-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15331-1-AP (MVD Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 15331-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15331-1-AP (MVD Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of A431 using 15331-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed A431 cells using 15331-1-AP (MVD antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HCT 116-Zellen, HepG2-Zellen, K-562-Zellen, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: humanes Kolongewebe, humanes Herzgewebe, humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 1 publications below

Produktinformation

15331-1-AP bindet in WB, IHC, IF, ELISA MVD und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MVD fusion protein Ag7255
• Vollständiger Name: mevalonate (diphospho) decarboxylase
• Berechnetes Molekulargewicht: 43 kDa
• Beobachtetes Molekulargewicht: 66-74 kDa, 45 kDa, 37 kDa
• GenBank-Zugangsnummer: BC000011
• Gene symbol: MVD
• Gene ID (NCBI): 4597
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The enzyme mevalonate pyrophosphate decarboxylase(MVD) catalyzes the conversion of mevalonate pyrophosphate into isopentenyl pyrophosphate. It is also named as MPD and as a unique enzyme in one of the early steps in cholesterol biosynthesis, MVD may be a useful target for drugs aimed at lowering serum cholesterol levels(PMID:8626466). The intracellular glycosylation does not contribute to the difference between the 45 and 37 kDa species of MVD. The native MVD has a molecular weight of 90 kDa that it consists of two identical subunits of 45 kDa and a 37 kDa protein is also found as a subunit of MVD and this type of MVD may be a 74 kDa.But the 37 kDa enzyme appeared only when the rats are fed the CP diet.(PMID:9348097).

Publikationen

Species	Application	Title
human	WB	J Hepatocell Carcinoma; miR-612 Enhances RSL3-Induced Ferroptosis of Hepatocellular Carcinoma Cells via Mevalonate Pathway; Authors - Kailin Xing
mouse	IHC	Cell Signal; Circ_0011058 facilitates proliferation, angiogenesis and radioresistance in papillary thyroid cancer cells by positively regulating YAP1 via acting as miR-335-5p sponge.; Authors - Zhenhua Zhang
mouse	WB	Curr Issues Mol Biol; Gigantol Improves Cholesterol Metabolism and Progesterone Biosynthesis in MA-10 Leydig Cells.; Authors - Audrey Basque
human	WB	Front Pharmacol; CCR9 overexpression promotes T-ALL progression by enhancing cholesterol biosynthesis; Authors - Muhammad Jamal

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

k (Verified Customer) (06-10-2021)

We always got good bands in western with this Ab

• Applications: Western Blot
• Primary Antibody Dilution: 1;1000