MVK Polyklonaler Antikörper

MVK Polyklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 12228-1-AP

Synonyme

LRBP, mevalonate kinase, MK, MVK, MVLK

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Galerie der Validierungsdaten

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  WB analysis using 12228-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12228-1-AP (MVK antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 12228-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 12228-1-AP (MVK antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse uterus using 12228-1-AP

  mouse uterus tissue were subjected to SDS PAGE followed by western blot with 12228-1-AP (MVK antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 12228-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 12228-1-AP (MVK antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse uterus using 12228-1-AP

  mouse uterus tissue were subjected to SDS PAGE followed by western blot with 12228-1-AP (MVK antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IF Staining of PC-3 using 12228-1-AP

  Immunofluorescent analysis of PC-3 cells using 12228-1-AP (MVK antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Caco-2-Zellen, HepG2-Zellen, Jurkat-Zellen, Maus-Uterusgewebe, PC-3-Zellen
• Erfolgreiche Detektion in IF: PC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below

Produktinformation

12228-1-AP bindet in WB, IF, ELISA MVK und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MVK fusion protein Ag2865
• Vollständiger Name: mevalonate kinase
• Berechnetes Molekulargewicht: 396 aa, 42 kDa
• Beobachtetes Molekulargewicht: 42 kDa
• GenBank-Zugangsnummer: BC016140
• Gene symbol: MVK
• Gene ID (NCBI): 4598
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MVK (Mevalonate kinase) is a 42 kDa cytoplasmic protein that belongs to the GHMP kinase family. Mevalonate kinase catalyzes the ATP-dependent phosphorylation of mevalonic acid to form mevalonate 5-phosphate. Defects in mevalonate kinase can cause mevalonic aciduria (MEVA). It is an accumulation of mevalonic acid which causes a variety of symptoms such as psychomotor retardation, dysmorphic features, cataracts, hepatosplenomegaly, lymphadenopathy, anemia, hypotonia, myopathy and ataxia.

Publikationen

Species	Application	Title
human	WB	J Hepatocell Carcinoma; miR-612 Enhances RSL3-Induced Ferroptosis of Hepatocellular Carcinoma Cells via Mevalonate Pathway; Authors - Kailin Xing
mouse	WB	Curr Issues Mol Biol; Gigantol Improves Cholesterol Metabolism and Progesterone Biosynthesis in MA-10 Leydig Cells.; Authors - Audrey Basque
human	WB	Exp Ther Med; Effects of mevalonate kinase interference on cell differentiation, apoptosis, prenylation and geranylgeranylation of human keratinocytes are attenuated by farnesyl pyrophosphate or geranylgeranyl pyrophosphate.; Authors - Min Li
mouse	WB	Toxicol In Vitro; Sinapic and ferulic acid phenethyl esters increase the expression of steroidogenic genes in MA-10 tumor Leydig cells; Authors - Audrey Basque