MVP/LRP Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

MVP/LRP Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(16)

Kat-Nr. : 16478-1-AP

Synonyme

MVP, LRP, VAULT1, Major vault protein, Lung resistance-related protein

---

• 

  WB analysis using 16478-1-AP

  Various cells were subjected to SDS PAGE followed by western blot with 16478-1-AP (MVP/LRP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 16478-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 16478-1-AP (MVP/LRP antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat lung using 16478-1-AP

  rat lung tissue were subjected to SDS PAGE followed by western blot with 16478-1-AP (MVP/LRP antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW264.7 using 16478-1-AP

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 16478-1-AP (MVP/LRP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 16478-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16478-1-AP (MVP/LRP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse lung using 16478-1-AP

  IP result of anti-MVP/LRP (IP:16478-1-AP, 4ug; Detection:16478-1-AP 1:500) with mouse lung tissue lysate 4000ug.

• 

  IHC staining of human lung cancer using 16478-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16478-1-AP (MVP/LRP antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 16478-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16478-1-AP (MVP/LRP antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human normal colon using 16478-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 16478-1-AP (MVP/LRP antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 16478-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 16478-1-AP (MVP/LRP antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 16478-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16478-1-AP (MVP/LRP antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 16478-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16478-1-AP (MVP/LRP antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human lung cancer using 16478-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16478-1-AP (MVP/LRP antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 16478-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16478-1-AP (MVP/LRP antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 16478-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16478-1-AP (MVP/LRP antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skin using 16478-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16478-1-AP (MVP/LRP antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin using 16478-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16478-1-AP (MVP/LRP antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 16478-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16478-1-AP (MVP/LRP antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 16478-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MVP/LRP antibody (16478-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, humanes Hirngewebe, Mauslungengewebe, Rattenlungengewebe, SH-SY5Y-Zellen
• Erfolgreiche IP: Mauslungengewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Nierengewebe, humanes Lungenkarzinomgewebe, humanes Hautgewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 13 publications below
• IHC: See 4 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

16478-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA MVP/LRP und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MVP/LRP fusion protein Ag9593
• Vollständiger Name: major vault protein
• Berechnetes Molekulargewicht: 893 aa, 99 kDa
• Beobachtetes Molekulargewicht: 99-104 kDa
• GenBank-Zugangsnummer: BC015623
• Gene symbol: LRP
• Gene ID (NCBI): 9961
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Vaults are the largest cellular ribonuclear protein complexes with a hollow barrel-like structure, and have been associated with the MDR phenotype. Major vault protein (MVP) is the main component of vaults and is presumed to be involved in MDR. MVP is identical with the human lung resistance protein (LRP), known to be overexpressed in multiple chemotherapy resistance models [PMID:23083532]. MVP has been associated to resistance to radiotherapy [4], probably due to its role in preventing apoptosis by inhibiting the COP-1/p53 axis [PMID: 12894554, 15994960].

Publikationen

Species	Application	Title
mouse	WB	Cell; The Mammalian Ribo-interactome Reveals Ribosome Functional Diversity and Heterogeneity.; Authors - Deniz Simsek
human	WB,IHC,IF,IP	Drug Resist Updat; CHD4 promotes acquired chemoresistance and tumor progression by activating the MEK/ERK axis; Authors - Jing Wu ; KD Validated
		Adv Sci (Weinh); Tryptophan As a New Member of RNA-Induced Silencing Complexes Prevents Colon Cancer Liver Metastasis; Authors - Fangyi Xu
human	WB	Nat Commun; MVP-mediated exosomal sorting of miR-193a promotes colon cancer progression.; Authors - Yun Teng ; KO Validated
human	WB	Front Immunol; Unraveling the role of Major Vault Protein as a novel immune-related biomarker that promotes the proliferation and migration in pancreatic adenocarcinoma; Authors - Xinyi Wu ; KD Validated
human	WB	Front Cell Dev Biol; Major Vault Protein (MVP) Associated With BRAF V600E Mutation Is an Immune Microenvironment-Related Biomarker Promoting the Progression of Papillary Thyroid Cancer via MAPK/ERK and PI3K/AKT Pathways.; Authors - Xubin Dong ; KD Validated