MX1 Rekombinanter Antikörper

MX1 Rekombinant Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2L19

Kat-Nr. : 82677-1-PBS

Synonyme

Interferon-regulated resistance GTP-binding protein MxA, Interferon-induced protein p78, Interferon-induced GTP-binding protein Mx1, N-terminally processed, Interferon-induced GTP-binding protein Mx1, IFI-78K

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  WB analysis using 82677-1-RR (same clone as 82677-1-PBS)

  Untreated and IFN alpha treated A549 cells, untreated and IFN alpha treated HepG2 cells were subjected to SDS PAGE followed by western blot with 82677-1-RR (MX1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82677-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 82677-1-RR (same clone as 82677-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 82677-1-RR (MX1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82677-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 82677-1-RR (same clone as 82677-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 82677-1-RR (MX1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82677-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 82677-1-RR (same clone as 82677-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 82677-1-RR (MX1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82677-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 82677-1-RR (same clone as 82677-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 82677-1-RR (MX1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82677-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 82677-1-RR (same clone as 82677-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using MX1 antibody (82677-1-RR, Clone: 2L19 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82677-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82677-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82677-1-RR against Human MX1 were performed. The affinity constant is 0.763 nM.

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Geprüfte Anwendungen

Produktinformation

82677-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA MX1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: MX1 fusion protein Ag4692
• Vollständiger Name: Myxovirus resistance protein 1
• Berechnetes Molekulargewicht: 662 aa, 76 kDa
• Beobachtetes Molekulargewicht: 75 kDa
• GenBank-Zugangsnummer: BC032602
• Gene symbol: MX1
• Gene ID (NCBI): 4599
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Mx1 (also known as MxA) is a GTPase that belongs to the Mx family of proteins, which are ubiquitous in eukaryotes and involved in the immune response to viral infections. The human Mx1 is localized in the cytoplasm interfering with the translation of viral proteins, whereas the mouse Mx1 accumulates in the nucleus and inhibits the primary transcription of viral RNA. Mx1 expression is induced by treatment with α/β IFN.