MXI1 Rekombinanter Antikörper

MXI1 Rekombinant Antikörper für WB, FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

242089D2

Kat-Nr. : 84733-5-PBS

Synonyme

242089D2, bHLHc11, Class C basic helix-loop-helix protein 11, MAD2, Max interacting protein 1

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  WB analysis using 84733-5-RR (same clone as 84733-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84733-5-RR (MXI1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84733-5-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP01509-3

  Sandwich ELISA standard curve of MP01509-3, Human MXI1 Recombinant Matched Antibody Pair - PBS only. 84733-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag3024. 84733-5-PBS was HRP conjugated as the detection antibody. Range: 0.625-40 ng/mL

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  Sample test of MP01509-3

  The mean MXI1 concentration was determined to be 20.19 ng/mL in HeLa cell extract based on a 1.0 mg/mL extract load.

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  FC experiment of HeLa using 84733-5-RR (same clone as 84733-5-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug MXI1 Recombinant antibody (84733-5-RR, Clone:242089D2) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84733-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84733-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84733-5-RR against Human MXI1 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

84733-5-PBS bindet in WB, FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test MXI1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: MXI1 fusion protein Ag3024
• Vollständiger Name: MAX interactor 1
• Berechnetes Molekulargewicht: 228 aa, 26 kDa
• Beobachtetes Molekulargewicht: 36 kDa
• GenBank-Zugangsnummer: BC012907
• Gene symbol: MXI1
• Gene ID (NCBI): 4601
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MXI1 is a transcription factor containing a basic helix-loop-helix leucine zipper (bHLHzip) and belongs to the Myc-Max-Mad transcriptional network. It is a transcriptional repressor thought to negatively regulate MYC function, and is therefore a potential tumor suppressor. This protein inhibits the transcriptional activity of MYC by competing for MAX, another basic helix-loop-helix protein that binds to MYC and is required for its function. Defects in this gene are frequently found in patients with prostate tumors.