MYBBP1A Polyklonaler Antikörper

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• KD/KO Validated

MYBBP1A Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14524-1-PBS

Synonyme

Myb binding protein 1A, Myb-binding protein 1A, PAP2

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  WB analysis using 14524-1-AP (same clone as 14524-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14524-1-AP (MYBBP1A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14524-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 14524-1-AP (same clone as 14524-1-PBS)

  WB result of MYBBP1A antibody (14524-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MYBBP1A transfected HEK-293 cells. This data was developed using the same antibody clone with 14524-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 14524-1-AP (same clone as 14524-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14524-1-AP (MYBBP1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14524-1-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 14524-1-AP (same clone as 14524-1-PBS)

  IP result of anti-MYBBP1A (IP:14524-1-AP, 4ug; Detection:14524-1-AP 1:500) with HEK-293 cells lysate 2800ug. This data was developed using the same antibody clone with 14524-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 14524-1-AP (same clone as 14524-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 14524-1-AP (MYBBP1A antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 14524-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 14524-1-AP (same clone as 14524-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 14524-1-AP (MYBBP1A antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 14524-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 14524-1-AP (same clone as 14524-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using MYBBP1A antibody 14524-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 14524-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

14524-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA MYBBP1A und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MYBBP1A fusion protein Ag6008
• Vollständiger Name: MYB binding protein (P160) 1a
• Berechnetes Molekulargewicht: 149 kDa
• Beobachtetes Molekulargewicht: 150-160 kDa
• GenBank-Zugangsnummer: BC050546
• Gene symbol: MYBBP1A
• Gene ID (NCBI): 10514
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The protooncogene MYB is predominantly expressed in immature hemopoietic cells where it has an essential role in hemopoietic cell proliferation and differentiation. Oncogenically activated forms of MYB is generally N- and/or C-terminal truncations of the normal MYB protein. Removal of the C terminus of MYB disrupts or deletes a region termed the negative regulatory domain (NRD), resulting in an increase in DNA binding, transactivation, and transformation by MYB. One feature of the NRD is a leucine zipper-like motif [PMID: 8302594]. Murine Myb-binding protein-1a (MYBBP1A), originally called P160, was identified by its ability to interact specifically with Myb via this leucine zipper-like motif. MYBBP1A modulates MYB activity upon binding to the MYB NRD [PMID: 10644447, 9447996].