MYBBP1A Monoklonaler Antikörper

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• KD/KO Validated

MYBBP1A Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1H2D10

Kat-Nr. : 67996-1-PBS

Synonyme

1H2D10, Myb binding protein 1A, Myb-binding protein 1A, PAP2

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  WB analysis using 67996-1-Ig (same clone as 67996-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67996-1-Ig (MYBBP1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67996-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 67996-1-Ig (same clone as 67996-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67996-1-Ig (MYBBP1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67996-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 67996-1-Ig (same clone as 67996-1-PBS)

  WB result of MYBBP1A antibody (67996-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MYBBP1A transfected HEK-293 cells. This data was developed using the same antibody clone with 67996-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 67996-1-Ig (same clone as 67996-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67996-1-Ig (MYBBP1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67996-1-PBS in a different storage buffer formulation.

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  WB analysis of 4T1 using 67996-1-Ig (same clone as 67996-1-PBS)

  4T1 cells were subjected to SDS PAGE followed by western blot with 67996-1-Ig (MYBBP1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67996-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 67996-1-Ig (same clone as 67996-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67996-1-Ig (MYBBP1A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67996-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67996-1-Ig (same clone as 67996-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MYBBP1A antibody (67996-1-Ig, Clone: 1H2D10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67996-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67996-1-Ig (same clone as 67996-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MYBBP1A antibody (67996-1-Ig, Clone: 1H2D10 ) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67996-1-PBS in a different storage buffer formulation.

• 

  IF Staining of NIH/3T3 using 67996-1-Ig (same clone as 67996-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using MYBBP1A antibody (67996-1-Ig, Clone: 1H2D10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67996-1-PBS in a different storage buffer formulation.

• 

  IF Staining of NIH/3T3 using 67996-1-Ig (same clone as 67996-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using MYBBP1A antibody (67996-1-Ig, Clone: 1H2D10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67996-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67996-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA MYBBP1A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: MYBBP1A fusion protein Ag6008
• Vollständiger Name: MYB binding protein (P160) 1a
• Berechnetes Molekulargewicht: 149 kDa
• Beobachtetes Molekulargewicht: 140 kDa
• GenBank-Zugangsnummer: BC050546
• Gene symbol: MYBBP1A
• Gene ID (NCBI): 10514
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The protooncogene MYB is predominantly expressed in immature hemopoietic cells where it has an essential role in hemopoietic cell proliferation and differentiation. Oncogenically activated forms of MYB is generally N- and/or C-terminal truncations of the normal MYB protein. Removal of the C terminus of MYB disrupts or deletes a region termed the negative regulatory domain (NRD), resulting in an increase in DNA binding, transactivation, and transformation by MYB. One feature of the NRD is a leucine zipper-like motif [PMID: 8302594]. Murine Myb-binding protein-1a (MYBBP1A), originally called P160, was identified by its ability to interact specifically with Myb via this leucine zipper-like motif. MYBBP1A modulates MYB activity upon binding to the MYB NRD [PMID: 10644447, 9447996].