MYBPC2 Polyklonaler Antikörper

MYBPC2 Polyklonal Antikörper für WB, IF-P, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF-P, IP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 25257-1-AP

Synonyme

MYBPCF, Fast MyBP C

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  WB analysis using 25257-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25257-1-AP (MYBPC2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse skeletal muscle using 25257-1-AP

  IP result of anti-MYBPC2 (IP:25257-1-AP, 4ug; Detection:25257-1-AP 1:40000) with mouse skeletal muscle tissue lysate 1040 ug.

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  IF Staining of mouse skeletal muscle using 25257-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using MYBPC2 antibody (25257-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Skelettmuskelgewebe, Ratten-Skelettmuskelgewebe
• Erfolgreiche IP: Maus-Skelettmuskelgewebe
• Erfolgreiche Detektion in IF-P: Maus-Skelettmuskelgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

25257-1-AP bindet in WB, IF-P, IP, ELISA MYBPC2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MYBPC2 fusion protein Ag19424
• Vollständiger Name: myosin binding protein C, fast type
• Berechnetes Molekulargewicht: 1141 aa, 128 kDa
• Beobachtetes Molekulargewicht: 130 kDa
• GenBank-Zugangsnummer: BC136389
• Gene symbol: MYBPC2
• Gene ID (NCBI): 4606
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MYBPC2, also known as MYBPCF, fMyBP-C, is a member of the myosin-binding protein C (MYBPC)family. MYBPC is a myosin-associated protein found in the cross-bridge-bearing zone, or C region, of A bands in striated muscle. MYBPC has three paralogs encoded by unique genes, including slow skeletal (MYBPC1), fast skeletal (MYBPC2), and cardiac (MYBPC3). Slow skeletal MyBP-C (sMyBP-C), fast skeletal MyBP-C (fMyBP-C), and cardiac MyBP-C (cMyBP-C) proteins are highly conserved with over 90% homology. They play unique muscle-specific structural and regulatory roles in actomyosin interactions and contractility in striated muscles, including both cardiac and skeletal muscles (PMID: 35832193, 23936073).