MYBPC3 Polyklonaler Antikörper

MYBPC3 Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 19977-1-PBS

Synonyme

Myosin-binding protein C, cardiac-type, MYBPC 3, C-protein, cardiac muscle isoform, Cardiac MyBP-C, Cardiac MyBP C

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  WB analysis using 19977-1-AP (same clone as 19977-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 19977-1-AP (MYBPC3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19977-1-PBS in a different storage buffer formulation.

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  WB analysis of human heart using 19977-1-AP (same clone as 19977-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 19977-1-AP (MYBPC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19977-1-PBS in a different storage buffer formulation.

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  WB analysis of rat heart using 19977-1-AP (same clone as 19977-1-PBS)

  rat heart tissue were subjected to SDS PAGE followed by western blot with 19977-1-AP (MYBPC3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19977-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 19977-1-AP (same clone as 19977-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 19977-1-AP (MYBPC3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19977-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 19977-1-AP (same clone as 19977-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 19977-1-AP (MYBPC3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19977-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse heart using 19977-1-AP (same clone as 19977-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 19977-1-AP (MYBPC3 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 19977-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse heart using 19977-1-AP (same clone as 19977-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 19977-1-AP (MYBPC3 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 19977-1-PBS in a different storage buffer formulation.

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  IF Staining of C2C12 using 19977-1-AP (same clone as 19977-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed C2C12 cells using MYBPC3 antibody (19977-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 19977-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

19977-1-PBS bindet in WB, IHC, IF/ICC, IF-P, Indirect ELISA MYBPC3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: myosin binding protein C, cardiac
• Berechnetes Molekulargewicht: 141 kDa
• Beobachtetes Molekulargewicht: 141-150 kDa
• GenBank-Zugangsnummer: NM_000256
• Gene symbol: MYBPC3
• Gene ID (NCBI): 4607
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MYBPC3 belongs to the immunoglobulin superfamily and MyBP family. MYBPC3 located in the crossbridge region of vertebrate striated muscle a bands. In vitro it binds MHC, F-actin and native thin filaments, and modifies the activity of actin-activated myosin ATPase. It may modulate muscle contraction or may play a more structural role. Defects in MYBPC3 are the cause of cardiomyopathy familial hypertrophic type 4 (CMH4). The antibody detects the full length form and the degradation bands around 28-54 kDa in SDS-PAGE.(PMID: 22076249)