MYBPC3 Monoklonaler Antikörper

MYBPC3 Monoklonal Antikörper für WB, IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3C8F8

Kat-Nr. : 67608-1-PBS

Synonyme

3C8F8, Cardiac MyBP C, Cardiac MyBP-C, C-protein, cardiac muscle isoform, MYBPC 3

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  WB analysis of mouse heart using 67608-1-Ig (same clone as 67608-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 67608-1-Ig (MYBPC3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67608-1-PBS in a different storage buffer formulation.

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  WB analysis of H9C2 using 67608-1-Ig (same clone as 67608-1-PBS)

  H9C2 cells were subjected to SDS PAGE followed by western blot with 67608-1-Ig (MYBPC3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67608-1-PBS in a different storage buffer formulation.

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  WB analysis of rabbit heart using 67608-1-Ig (same clone as 67608-1-PBS)

  rabbit heart tissue were subjected to SDS PAGE followed by western blot with 67608-1-Ig (MYBPC3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67608-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig heart using 67608-1-Ig (same clone as 67608-1-PBS)

  pig heart tissue were subjected to SDS PAGE followed by western blot with 67608-1-Ig (MYBPC3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67608-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 67608-1-Ig (same clone as 67608-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 67608-1-Ig (MYBPC3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67608-1-PBS in a different storage buffer formulation.

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  IHC staining of rat heart using 67608-1-Ig (same clone as 67608-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 67608-1-Ig (MYBPC3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67608-1-PBS in a different storage buffer formulation.

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  IF Staining of rat heart using 67608-1-Ig (same clone as 67608-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed rat heart tissue using MYBPC3 antibody (67608-1-Ig, Clone: 3C8F8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67608-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67608-1-PBS bindet in WB, IHC, IF-P, Indirect ELISA MYBPC3 und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: MYBPC3 fusion protein Ag15848
• Vollständiger Name: myosin binding protein C, cardiac
• Berechnetes Molekulargewicht: 1274 aa, 141 kDa
• Beobachtetes Molekulargewicht: 140-150 kDa
• GenBank-Zugangsnummer: BC151211
• Gene symbol: MYBPC3
• Gene ID (NCBI): 4607
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MYBPC3 belongs to the immunoglobulin superfamily and MyBP family. MYBPC3 located in the crossbridge region of vertebrate striated muscle a bands. In vitro it binds MHC, F-actin and native thin filaments, and modifies the activity of actin-activated myosin ATPase. It may modulate muscle contraction or may play a more structural role. Defects in MYBPC3 are the cause of cardiomyopathy familial hypertrophic type 4 (CMH4).