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- KD/KO Validated
c-MYC Polyklonaler Antikörper
c-MYC Polyklonal Antikörper für FC, IF, IP, WB, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human und mehr (4)
Anwendung
WB, IP, IF, FC, EMSA, CoIP, ChIP, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 10828-1-AP
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | HT-29-Zellen, A549-Zellen, BxPC-3-Zellen, HeLa-Zellen, HepG2-Zellen, HL-60-Zellen, humanes Plazenta-Gewebe, Jurkat-Zellen, Jurkat-Zellen und Raji-Zellen., Raji-Zellen, SH-SY5Y-Zellen, SW 1990-Zellen |
Erfolgreiche IP | MCF-7-Zellen |
Erfolgreiche Detektion in IF | Saos-2 cells, HEK-293-Zellen, U2OS-Zellen |
Erfolgreiche Detektion in FC | HeLa-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
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Western Blot (WB) | WB : 1:2000-1:12000 |
Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunfluoreszenz (IF) | IF : 1:200-1:800 |
Durchflusszytometrie (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
KD/KO | See 21 publications below |
WB | See 516 publications below |
IF | See 33 publications below |
IP | See 19 publications below |
CoIP | See 14 publications below |
ChIP | See 10 publications below |
Produktinformation
10828-1-AP bindet in WB, IP, IF, FC, EMSA, CoIP, ChIP, ELISA c-MYC und zeigt Reaktivität mit human
Getestete Reaktivität | human |
In Publikationen genannte Reaktivität | human, Hausschwein, Huhn, Maus, Ratte |
Wirt / Isotyp | Kaninchen / IgG |
Klonalität | Polyklonal |
Typ | Antikörper |
Immunogen | c-MYC fusion protein Ag1263 |
Vollständiger Name | v-myc myelocytomatosis viral oncogene homolog (avian) |
Berechnetes Molekulargewicht | 49 kDa |
Beobachtetes Molekulargewicht | 62-65 kDa, 50 kDa |
GenBank-Zugangsnummer | BC000141 |
Gene symbol | MYC |
Gene ID (NCBI) | 4609 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Antigen-Affinitätsreinigung |
Lagerungspuffer | PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3. |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
Function
c-Myc (also known as Myc), together with l-Myc and n-Myc, belongs to the Myc family of transcription factors. c-Myc has a basic helix-loop-helix leucine zipper domain and through heterodimerization can bind and regulate the transcriptional activity of genes, either by repression or activation. It is a key player in the regulation of cell growth and cell cycle progression and acts as a proto-oncogene.
Tissue specificity
c-Myc is ubiquitously expressed in almost all cell types and its expression positively correlates with tissue proliferative capacity. c-Myc is also expressed during embryogenesis and is upregulated in many cancer types.
Involvement in disease
· Upregulated in many cancer types, especially in aggressive, poorly differentiated tumors.
· Mutations in the MYC gene and breakpoint translocations within the MYC gene cause Burkitt lymphoma.
Isoforms
There are 3 different isoforms of c-Myc: c-Myc1, c-Myc2, and c-MycS (PMID: 16260605). They differ in molecular size, can be preferentially expressed during cell growth, and are reported to be functionally distinct.
Post-translational modifications
c-Myc is subject to various post-translational modifications, including phosphorylation, acetylation, and ubiquitinylation (PMID: 16987807), which regulate its activity.
Cellular localization
c-Myc localizes to the nucleus but can also be present in the cytoplasm of certain cancer types.
Protokolle
Produktspezifische Protokolle | |
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WB protocol for c-MYC antibody 10828-1-AP | Protokoll herunterladen |
IF protocol for c-MYC antibody 10828-1-AP | Protokoll herunterladen |
IP protocol for c-MYC antibody 10828-1-AP | Protokoll herunterladen |
Standard-Protokolle | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
Signal Transduct Target Ther MDIG-mediated H3K9me3 demethylation upregulates Myc by activating OTX2 and facilitates liver regeneration
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Signal Transduct Target Ther Overexpression of CIP2A is associated with poor prognosis in multiple myeloma. | ||
Gastroenterology Nucleolar HEATR1 upregulated by mTORC1 signaling promotes hepatocellular carcinoma growth by dominating ribosome biogenesis and proteome homeostasis | ||
Cancer Cell p53 Is a Master Regulator of Proteostasis in SMARCB1-Deficient Malignant Rhabdoid Tumors.
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Cell Metab Efferocytosis induces macrophage proliferation to help resolve tissue injury.
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Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Molly (Verified Customer) (05-02-2023) | The antibody worked well for western blotting following the recommended protocol.
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FH Anna-Maria (Verified Customer) (08-13-2019) | This antibody worked as expected for western blotting
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FH Christine (Verified Customer) (11-13-2018) | The antibody worked better than the Abcam c-Myc antibody. However, I did not get clear nuclear staining but rather a mix of nuclear staining with cytoplasmic staining. This could be due non-optimized conditions.
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FH Claudio (Verified Customer) (12-14-2017) | We tested the antibody in IHC/IFL on mouse brain tumors that showed c-myc upregulation at the mRNA level.
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