MYC tag Monoklonaler Antikörper

MYC tag Monoklonal Antikörper für WB, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

rekombinanten Protein

Anwendung

WB, IF/ICC, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

CloneNo.

1A5A2

Kat-Nr. : 60003-2-PBS

Synonyme

myc, EQKLISEEDL, MYC-tag

---

• 

  WB analysis of recombinant proein using 60003-2-Ig (same clone as 60003-2-PBS)

  Western blot of Myc-tagged fusion protein with anti-Myc-tag (60003-2-Ig) at various dilutions. This data was developed using the same antibody clone with 60003-2-PBS in a different storage buffer formulation.

• 

  WB analysis using 60003-2-Ig (same clone as 60003-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60003-2-Ig (MYC tag antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60003-2-PBS in a different storage buffer formulation.

• 

  IP experiment of Transfected HEK-293 using 60003-2-Ig (same clone as 60003-2-PBS)

  IP result of anti-MYC tag (IP:60003-2-Ig, 4ug; Detection:60003-2-Ig 1:10000) with Transfected HEK-293 cells lysate 300ug. This data was developed using the same antibody clone with 60003-2-PBS in a different storage buffer formulation.

• 

  IF Staining of Transfected HEK-293 using 60003-2-Ig (same clone as 60003-2-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using MYC tag antibody (60003-2-Ig, Clone: 1A5A2 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60003-2-PBS in a different storage buffer formulation.

• 

  FC experiment of HEK-293 transfected with GFP-MYC using 60003-2-Ig (same clone as 60003-2-PBS)

  1x10^6 HEK-293 transfected with GFP-MYC were intracellularly stained with 0.25 ug MYC tag Monoclonal antibody (60003-2-Ig, Clone:1A5A2) and Multi-rAb CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L)(RGAM005). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 60003-2-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

60003-2-PBS bindet in WB, IF/ICC, FC (Intra), IP, ELISA MYC tag und zeigt Reaktivität mit rekombinanten Protein

• Getestete Reaktivität: rekombinanten Protein
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: Myc tag
• Gene symbol: Myc tag
• Gene ID (NCBI): 99
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The c-Myc tag corresponds to amino acid residues(EQKLISEEDL) of the human c-Myc protein. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. Myc-Tag mouse mAb detects recombinant proteins containing the Myc tag. The antibody recognizes the Myc-tag EQKLISEEDL fused to either the amino- or carboxy- terminus of targeted proteins.