MYC tag Rekombinanter Antikörper

MYC tag Rekombinant Antikörper für WB, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

rekombinanten Protein

Anwendung

WB, IF/ICC, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

CloneNo.

241611D1

Kat-Nr. : 80469-2-RR

Synonyme

241611D1

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  WB analysis of Transfected HEK-293T using 80469-2-RR

  Transfected HEK-293T cells were subjected to SDS PAGE followed by western blot with 80469-2-RR (MYC tag antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

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  IP experiment of Transfected HEK-293 using 80469-2-RR

  IP result of anti-MYC tag (IP:80469-2-RR, 4ug; Detection:80469-2-RR 1:5000) with Transfected HEK-293 cells lysate 1600 ug.

• 

  IF Staining of Transfected HEK-293 using 80469-2-RR

  Immunofluorescent analysis of (4% PFA) fixed Transfected HEK-293 cells using MYC tag antibody (80469-2-RR, Clone: 241611D1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  FC experiment of Transfected HEK-293 using 80469-2-RR

  1x10^6 Transfected HEK-293 cells were intracellularly stained with 0.25 ug MYC tag Recombinant antibody (80469-2-RR, Clone:241611D1) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), and 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 80469-2-RR

  Biolayer interferometry (BLl) kinetic assays of 80469-2-RR against MYC tag were performed. The affinity constant is 0.134 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Transfected HEK-293T cells
• Erfolgreiche IP: Transfizierte HEK-293-Zellen
• Erfolgreiche Detektion in IF/ICC: Transfizierte HEK-293-Zellen
• Erfolgreiche Detektion in FC (Intra): Transfizierte HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:20000-1:100000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:125-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

80469-2-RR bindet in WB, IF/ICC, FC (Intra), IP, ELISA MYC tag und zeigt Reaktivität mit rekombinanten Protein

• Getestete Reaktivität: rekombinanten Protein
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: MYC tag fusion protein Ag9409
• Vollständiger Name: Myc tag
• Gene symbol: Myc tag
• Gene ID (NCBI): 99
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The myc-tag is a short synthetic polypeptide sequence derived from c-MYC protein that can be added to recombinant proteins to enable isolation and study when an antibody is not available. This antibody recognizes the MYC tag EQKLISEEDL. MYC tag is a part of c-MYC protein. So there's weaker 62-65 kDa bands for endogenous c-MYC protein. What is the molecular weight of myc? 1203Da: the ten amino acid myc tag sequence is EQKLISEEDL (in single letter code). What are the applications for myc tag? The addition of the myc tag to a particular protein can be useful when an antibody is not available to the protein of interest. Using recombinant DNA technology, the myc tag can be fused to the protein and then an antibody against the myc tag can be used to probe (PMID: 24490106). This is a reliable method and can be used in a number of different techniques, including purification using chromatography, tracking the protein in localization studies using immunofluorescence, or quantifying levels using Western Blot (PMID: 24490106). What is the structure of myc tag? The c-myc gene from which this tag is derived has a molecular weight of 49kDa, but the myc tag represents only a small portion of the C-terminus of this gene. The short polypeptide sequence can be fused to the N-terminus or the C-terminus of any protein without influencing function, although it is advised to avoid fusing it to a secretory signal. A cleavage site behind this tag is also sometimes engineered to allow removal with a specific protease.