MYCBP Polyklonaler Antikörper

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• KD/KO Validated

MYCBP Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF/ICC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

Kat-Nr. : 12022-1-AP

Synonyme

AMY 1, AMY1, AMY-1, Associate of Myc 1, c-Myc-binding protein

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  WB analysis of human heart using 12022-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 12022-1-AP (MYCBP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 12022-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12022-1-AP (MYCBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 12022-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12022-1-AP (MYCBP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human lung using 12022-1-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 12022-1-AP (MYCBP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 12022-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 12022-1-AP (MYCBP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 12022-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12022-1-AP (MYCBP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 12022-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12022-1-AP (MYCBP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 12022-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 12022-1-AP (MYCBP antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 12022-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12022-1-AP (MYCBP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 12022-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MYCBP antibody (12022-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Herzgewebe, A549-Zellen, HEK-293-Zellen, humanes Nierengewebe, humanes Lungengewebe, humanes Plazenta-Gewebe
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe, Maus-Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

12022-1-AP bindet in WB, IHC, IF/ICC, CoIP, ELISA MYCBP und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MYCBP fusion protein Ag2651
• Vollständiger Name: c-myc binding protein
• Berechnetes Molekulargewicht: 103 aa, 12 kDa
• Beobachtetes Molekulargewicht: 12 kDa
• GenBank-Zugangsnummer: BC008686
• Gene symbol: MYCBP
• Gene ID (NCBI): 26292
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MYC binding protein(MYCBP), encoded by MYCBP gene, binds to N-terminal region of MYC, and then MYC can activate E box-dependent transcription. Upon increasing expression of MYC , MYCBP translocates into the nucleus in the S phase of the cell cycle. MYCBP may involve in spermatogenesis, since it's found to be interacted with AKAP84/149 in the mitochondria in somatic cells and sperm. It highly expressed in heart, placenta, pancreas,skeletal muscle and kidney, but low in lung. This is a rabbit polyclonal antibody raised against full-length MYCBP of human origin.

Publikationen

Species	Application	Title
human	IF	Mol Cell; Spatiotemporal Proteomic Analysis of Stress Granule Disassembly Using APEX Reveals Regulation by SUMOylation and Links to ALS Pathogenesis.; Authors - Hagai Marmor-Kollet
human	WB	Mol Cell; An Interaction Landscape of Ubiquitin Signaling.; Authors - Xiaofei Zhang
mouse	WB	Acta Physiol (Oxf); A dual role of miR-22 in rhabdomyolysis-induced acute kidney injury.; Authors - S Mathia
mouse	WB	J Cell Mol Med; Mesenchymal stem cell-secreted extracellular vesicles carrying TGF-β1 up-regulate miR-132 and promote mouse M2 macrophage polarization.; Authors - Yongqi Wang
human	WB	Oncol Lett; Overexpression of MYC binding protein promotes invasion and migration in gastric cancer.; Authors - Lijie Gong ; KD Validated
mouse	IF	Cell Mol Life Sci; CFAP65 is essential for C2a projection integrity in axonemes: implications for organ-specific ciliary dysfunction and infertility; Authors - Jinyi Chen