MYCBP Polyklonaler Antikörper

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• KD/KO Validated

MYCBP Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12022-1-PBS

Synonyme

c-Myc-binding protein, Associate of Myc 1, AMY-1, AMY1, AMY 1

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  WB analysis of human heart using 12022-1-AP (same clone as 12022-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 12022-1-AP (MYCBP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12022-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 12022-1-AP (same clone as 12022-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12022-1-AP (MYCBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12022-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human kidney using 12022-1-AP (same clone as 12022-1-PBS)

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12022-1-AP (MYCBP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12022-1-PBS in a different storage buffer formulation.

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  WB analysis of human lung using 12022-1-AP (same clone as 12022-1-PBS)

  human lung tissue were subjected to SDS PAGE followed by western blot with 12022-1-AP (MYCBP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12022-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human placenta using 12022-1-AP (same clone as 12022-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 12022-1-AP (MYCBP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12022-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 12022-1-AP (same clone as 12022-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12022-1-AP (MYCBP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12022-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 12022-1-AP (same clone as 12022-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12022-1-AP (MYCBP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12022-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 12022-1-AP (same clone as 12022-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12022-1-AP (MYCBP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12022-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse colon using 12022-1-AP (same clone as 12022-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 12022-1-AP (MYCBP antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12022-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 12022-1-AP (same clone as 12022-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MYCBP antibody (12022-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 12022-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

12022-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA MYCBP und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MYCBP fusion protein Ag2651
• Vollständiger Name: c-myc binding protein
• Berechnetes Molekulargewicht: 103 aa, 12 kDa
• Beobachtetes Molekulargewicht: 12 kDa
• GenBank-Zugangsnummer: BC008686
• Gene symbol: MYCBP
• Gene ID (NCBI): 26292
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MYC binding protein(MYCBP), encoded by MYCBP gene, binds to N-terminal region of MYC, and then MYC can activate E box-dependent transcription. Upon increasing expression of MYC , MYCBP translocates into the nucleus in the S phase of the cell cycle. MYCBP may involve in spermatogenesis, since it's found to be interacted with AKAP84/149 in the mitochondria in somatic cells and sperm. It highly expressed in heart, placenta, pancreas,skeletal muscle and kidney, but low in lung. This is a rabbit polyclonal antibody raised against full-length MYCBP of human origin.