MYCBP2 Polyklonaler Antikörper

MYCBP2 Polyklonal Antikörper für IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IHC, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 27951-1-AP

Synonyme

KIAA0916, MYC binding protein 2, PAM, Pam/highwire/rpm 1 protein, Protein associated with Myc

---

• 

  IHC staining of human brain using 27951-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 27951-1-AP (MYCBP2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 27951-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 27951-1-AP (MYCBP2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 27951-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 27951-1-AP (MYCBP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 27951-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 27951-1-AP (MYCBP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 27951-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27951-1-AP (MYCBP2 antibody) at dilution of 1:200 (under 0x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 27951-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27951-1-AP (MYCBP2 antibody) at dilution of 1:200 (under 0x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of U2OS using 27951-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using MYCBP2 antibody (27951-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  FC experiment of U2OS using 27951-1-AP

  1x10^6 U2OS cells were intracellularly stained with 0.4 ug MYCBP2 Polyclonal antibody (27951-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Hirngewebe, Maushirngewebe, Rattenhirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: U2OS-Zellen
• Erfolgreiche Detektion in FC (Intra): U2OS-Zellen

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 2 publications below

Produktinformation

27951-1-AP bindet in IHC, IF/ICC, FC (Intra), ELISA MYCBP2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MYCBP2 fusion protein Ag27228
• Vollständiger Name: MYC binding protein 2
• Berechnetes Molekulargewicht: 510 kDa
• GenBank-Zugangsnummer: NM_015057
• Gene symbol: MYCBP2
• Gene ID (NCBI): 23077
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	IHC	Front Immunol; MYCBP2 expression correlated with inflammatory cell infiltration and prognosis immunotherapy in thyroid cancer patients; Authors - Guilin Wang
human	IHC	Arch Biochem Biophys; Exosomes from human umbilical cord mesenchymal stem cells protect aortas in Db/db mice characterized by combination of metabolomics and proteomics; Authors - Ming-Ying Ling