MYCBP2 Polyklonaler Antikörper

MYCBP2 Polyklonal Antikörper für IHC, IF/ICC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IHC, IF/ICC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 27951-1-PBS

Synonyme

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  IHC staining of human brain using 27951-1-AP (same clone as 27951-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 27951-1-AP (MYCBP2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27951-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 27951-1-AP (same clone as 27951-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 27951-1-AP (MYCBP2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27951-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 27951-1-AP (same clone as 27951-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 27951-1-AP (MYCBP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27951-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 27951-1-AP (same clone as 27951-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 27951-1-AP (MYCBP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27951-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 27951-1-AP (same clone as 27951-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27951-1-AP (MYCBP2 antibody) at dilution of 1:200 (under 0x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27951-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 27951-1-AP (same clone as 27951-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27951-1-AP (MYCBP2 antibody) at dilution of 1:200 (under 0x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27951-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 27951-1-AP (same clone as 27951-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using MYCBP2 antibody (27951-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 27951-1-PBS in a different storage buffer formulation.

• 

  FC experiment of U2OS using 27951-1-AP (same clone as 27951-1-PBS)

  1x10^6 U2OS cells were intracellularly stained with 0.4 ug MYCBP2 Polyclonal antibody (27951-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 27951-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

27951-1-PBS bindet in IHC, IF/ICC, FC (Intra), Indirect ELISA MYCBP2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MYCBP2 fusion protein Ag27228
• Vollständiger Name: MYC binding protein 2
• Berechnetes Molekulargewicht: 510 kDa
• GenBank-Zugangsnummer: NM_015057
• Gene symbol: MYCBP2
• Gene ID (NCBI): 23077
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.