MYCBPAP Polyklonaler Antikörper

MYCBPAP Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 12763-1-AP

Synonyme

AMAP-1, AMAP1, AMAP 1, AMAM-1, AMAM1

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  WB analysis of human placenta using 12763-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 12763-1-AP (MYCBPAP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 12763-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12763-1-AP (MYCBPAP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 12763-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12763-1-AP (MYCBPAP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 12763-1-AP

  Immunofluorescent analysis of HepG2 cells, using MYCBPAP antibody 12763-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of HUVEC using 12763-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using MYCBPAP antibody (12763-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Plazenta-Gewebe
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HUVEC-Zellen, HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

12763-1-AP bindet in WB, IHC, IF/ICC, ELISA MYCBPAP und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MYCBPAP fusion protein Ag3419
• Vollständiger Name: MYCBP associated protein
• Berechnetes Molekulargewicht: 947 aa, 108 kDa
• Beobachtetes Molekulargewicht: 108 kDa
• GenBank-Zugangsnummer: BC028393
• Gene symbol: MYCBPAP
• Gene ID (NCBI): 84073
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MYCBPAP, or MYCBP associated protein, is a protein encoded by the human MYCBPAP gene. It is primarily expressed in the testis and is involved in spermatogenesis, the process of forming sperm cells. The protein may also play a role in synaptic processes, which are essential for neuronal signaling. MYCBPAP is predicted to be an intracellular protein with molecular functions including clathrin binding, phospholipid binding, and protein binding. It is associated with biological processes such as cell differentiation, chemical synaptic transmission, endocytosis, and spermatogenesis.

Publikationen

Species	Application	Title
mouse	WB	Sci China Life Sci; Homozygous deleterious variants in MYCBPAP induce asthenoteratozoospermia involving abnormal acrosome biogenesis, manchette structure and sperm tail assembly in humans and mice; Authors - Yiling Zhou